{"product_id":"gal4-reporter-lentivirus-bhv19400034","title":"GAL4 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eGAL4 is a transcription factor from yeast that binds a defined DNA sequence called the upstream activating sequence (UAS). Because mammalian cells lack endogenous GAL4 and UAS elements, the GAL4-UAS system functions as an orthogonal, low-background transcriptional switch and is a foundational tool in molecular biology. In the mammalian two-hybrid assay, a protein of interest is fused to the GAL4 DNA-binding domain and a candidate partner is fused to the VP16 activation domain; only if the two proteins interact is the activation domain recruited to the UAS, switching on reporter expression. This design provides a sensitive, quantitative readout of protein-protein interactions in living cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe GAL4 Reporter Lentivirus is a lentiviral reporter system for the rapid readout of protein-protein interactions, suitable for mammalian two-hybrid assays. The construct contains five tandem repeats of the GAL4 UAS upstream of a fluorescent (GFP or RFP) or luminescent reporter, allowing convenient detection by fluorescence microscopy, flow cytometry, or luminometry. Reporter activity is produced in response to a GAL4 DNA-binding-domain fusion protein and is strongly enhanced when an interacting VP16 activation-domain fusion is present. Antibiotic selection markers (puromycin or blasticidin) support establishment of stable reporter cell lines. The system is used to detect and quantify protein-protein interactions and to screen interaction modulators. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, Firefly Luc, GFP + Firefly Luc, mCherry, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the GAL4-DBD for PPI and two-hybrid assay transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251620634989,"sku":"LTV-0038-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310898110829,"sku":"LTV-0038-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310898143597,"sku":"LTV-0038-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310898176365,"sku":"LTV-0038-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310898209133,"sku":"LTV-0038-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310898241901,"sku":"LTV-0038-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310898274669,"sku":"LTV-0038-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0038-GAL4-Reporter-Fig.jpg?v=1776934148","url":"https:\/\/www.ebiohippo.com\/products\/gal4-reporter-lentivirus-bhv19400034","provider":"BioHippo","version":"1.0","type":"link"}