{"product_id":"galnt12-antibody-polypeptide-n-acetylgalactosaminyltransferase-12-bha17135700","title":"GALNT12 Antibody \/ Polypeptide N-acetylgalactosaminyltransferase 12","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGALNT12 Antibody \/ Polypeptide N-acetylgalactosaminyltransferase 12 is a anti-GALNT12 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GALNT12\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGALNT12 antibody detects Polypeptide N-acetylgalactosaminyltransferase 12, an enzyme involved in initiating mucin-type O-linked glycosylation of proteins. Encoded by the GALNT12 gene on chromosome 9q22.33, this Golgi-resident glycosyltransferase catalyzes the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to serine or threonine residues on target proteins, forming the Tn antigen and enabling further elongation of O-glycan structures. GALNT12 plays a crucial role in regulating protein stability, secretion, and cell-cell communication through glycosylation control.\u003cbr\u003e\u003cbr\u003eStructurally, GALNT12 contains a catalytic domain typical of the GalNAc-transferase family and a ricin-like lectin domain that mediates substrate recognition. It preferentially glycosylates substrates involved in epithelial integrity and mucin production. GALNT12 is expressed in gastrointestinal tissues and epithelial linings, where it influences mucin biosynthesis, cell adhesion, and signaling. Its activity affects the glycoprotein composition of the extracellular matrix and epithelial surfaces.\u003cbr\u003e\u003cbr\u003eThe GALNT12 antibody is widely used in glycobiology, cancer, and epithelial research to study O-glycosylation and mucin biosynthesis. Western blot analysis identifies a 60 kilodalton band corresponding to GALNT12, while immunofluorescence reveals perinuclear staining consistent with Golgi localization. This antibody is instrumental in evaluating changes in glycosylation during differentiation, inflammation, and tumorigenesis.\u003cbr\u003e\u003cbr\u003eMutations in GALNT12 are associated with colorectal cancer susceptibility, where altered O-glycosylation patterns affect cell adhesion and immune recognition. Dysregulated GALNT12 expression may lead to incomplete mucin glycosylation, contributing to tumor progression and epithelial dysfunction. The GALNT12 antibody supports research into glycosylation-dependent signaling, epithelial homeostasis, and cancer pathogenesis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GALNT12 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GALNT12 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GALNT12 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GALNT12 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GALNT12+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047304094061,"sku":"FY12798","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_abee63af-748b-4dc2-8055-929331a7efe4.jpg?v=1782237051","url":"https:\/\/www.ebiohippo.com\/products\/galnt12-antibody-polypeptide-n-acetylgalactosaminyltransferase-12-bha17135700","provider":"BioHippo","version":"1.0","type":"link"}