{"product_id":"galt-antibody-galactose-1-phosphate-uridylyltransferase-bha17135880","title":"GALT Antibody \/ Galactose-1-phosphate uridylyltransferase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGALT Antibody \/ Galactose-1-phosphate uridylyltransferase is a anti-GALT Rabbit antibody Recombinant Rabbit Monoclonal clone 29G81 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GALT\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 29G81, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGALT antibody detects Galactose-1-phosphate uridylyltransferase, encoded by the GALT gene. This enzyme plays an essential role in the Leloir pathway of galactose metabolism, where it catalyzes the transfer of uridylyl groups between UDP glucose and galactose-1-phosphate to form UDP galactose and glucose-1-phosphate. This reaction is central to the proper metabolism of dietary galactose, and deficiency in GALT activity leads to classic galactosemia, an inherited metabolic disorder. GALT antibody allows researchers to study enzyme expression, localization, and regulation in both normal physiology and disease contexts.\u003cbr\u003e\u003cbr\u003eGalactose-1-phosphate uridylyltransferase is widely expressed in liver, kidney, and other metabolically active tissues. Research using GALT antibody has revealed that enzyme activity is tightly regulated, as accumulation of galactose-1-phosphate is toxic to cells. In classic galactosemia, mutations in the GALT gene result in absent or reduced activity, causing life threatening complications in infants such as liver failure, sepsis, and developmental delays. Early detection and dietary management are crucial, but long term complications still occur, making GALT antibody valuable in research aimed at improving therapies and understanding pathophysiology.\u003cbr\u003e\u003cbr\u003eBeyond metabolic disorders, GALT has been studied in cancer and broader metabolic regulation. Alterations in carbohydrate metabolism are a hallmark of tumor cells, and studies using GALT antibody have suggested that reduced GALT activity may influence cancer cell growth through altered glycosylation and signaling pathways. In addition, polymorphisms in the GALT gene have been examined in population studies for their potential links to reproductive outcomes and susceptibility to metabolic stress. These investigations underscore the enzyme's relevance beyond rare genetic disease.\u003cbr\u003e\u003cbr\u003eGALT antibody is widely used in western blotting, immunohistochemistry, and enzymatic activity assays. Western blotting confirms protein levels in cell and tissue lysates, while immunohistochemistry highlights tissue specific distribution. Functional assays combined with GALT antibody detection assess enzymatic activity under different experimental conditions, providing a comprehensive view of GALT regulation. These approaches make GALT antibody a powerful tool for metabolic research, clinical investigation, and biomarker development.\u003cbr\u003e\u003cbr\u003eBy supplying validated GALT antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GALT - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GALT - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GALT - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GALT - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GALT+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047308124525,"sku":"FY12978","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f55828aa-1c7d-417d-9335-ffda4c1ca29d.jpg?v=1772019370","url":"https:\/\/www.ebiohippo.com\/products\/galt-antibody-galactose-1-phosphate-uridylyltransferase-bha17135880","provider":"BioHippo","version":"1.0","type":"link"}