{"product_id":"gamt-antibody-guanidinoacetate-n-methyltransferase-bha17135549","title":"GAMT Antibody \/ Guanidinoacetate N-methyltransferase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGAMT Antibody \/ Guanidinoacetate N-methyltransferase is a anti-GAMT Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GAMT\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGAMT antibody detects Guanidinoacetate N-methyltransferase, an enzyme that catalyzes the final step in the biosynthesis of creatine, a key molecule for cellular energy storage and transfer. GAMT converts guanidinoacetate and S-adenosyl-L-methionine into creatine and S-adenosyl-L-homocysteine, a critical reaction for maintaining high-energy phosphate metabolism in muscle and neural tissues. The GAMT antibody is widely used in metabolism, neurology, and biochemical research to study creatine biosynthesis, methylation pathways, and energy regulation.\u003cbr\u003e\u003cbr\u003eGAMT is encoded by the GAMT gene located on human chromosome 19p13.3. The protein is approximately 236 amino acids long and localizes to the cytoplasm and mitochondria of metabolically active cells such as skeletal muscle fibers, hepatocytes, and neurons. GAMT belongs to the family of methyltransferases and functions as a homodimer, with each subunit contributing to catalytic activity and substrate specificity.\u003cbr\u003e\u003cbr\u003eThe GAMT antibody detects a 28 kilodalton protein by western blot and shows cytosolic and mitochondrial localization by immunofluorescence microscopy. Creatine synthesized by GAMT is essential for buffering cellular ATP levels through the phosphocreatine shuttle system, particularly in tissues with fluctuating energy demands. In the brain, creatine supports neuronal excitability and synaptic transmission, while in muscle, it sustains rapid contraction and recovery.\u003cbr\u003e\u003cbr\u003eDeficiency of GAMT causes guanidinoacetate methyltransferase deficiency, a rare inborn error of metabolism characterized by creatine depletion in the brain, developmental delay, seizures, and movement disorders. Accumulation of guanidinoacetate is neurotoxic, leading to oxidative stress and neurotransmission imbalance. Early diagnosis through enzyme assays or molecular testing is critical, as dietary creatine supplementation can mitigate neurological symptoms.\u003cbr\u003e\u003cbr\u003eBeyond its metabolic role, GAMT activity influences methylation capacity, homocysteine metabolism, and nitric oxide synthesis, linking it to broader aspects of cellular function. Altered GAMT expression has been associated with cardiovascular disease, neurodegeneration, and aging due to impaired energy homeostasis and methyl donor imbalance.\u003cbr\u003e\u003cbr\u003eBecause GAMT is central to energy metabolism and methyl group regulation, it serves as an important biomarker for studying metabolic diseases and neurological function.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GAMT - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GAMT - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GAMT - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GAMT - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GAMT+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047295181165,"sku":"FY12646","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ecfa40a8-caef-40c8-a1e2-55f4cd674fa3.jpg?v=1782237027","url":"https:\/\/www.ebiohippo.com\/products\/gamt-antibody-guanidinoacetate-n-methyltransferase-bha17135549","provider":"BioHippo","version":"1.0","type":"link"}