{"product_id":"gar1-antibody-h-aca-ribonucleoprotein-complex-subunit-1-nola1-bha17136330","title":"GAR1 Antibody \/ H\/ACA ribonucleoprotein complex subunit 1 \/ NOLA1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGAR1 Antibody \/ H\/ACA ribonucleoprotein complex subunit 1 \/ NOLA1 is a anti-GAR1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), Immunofluorescence (IF), Flow cytometry (FACS), Immunoprecipitation (IP), IHC-P with listed reactivity in Human. Reported localization: Nucleolus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GAR1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IF, FACS, IP, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGAR1 (H\/ACA ribonucleoprotein complex subunit 1) is a highly conserved nucleolar protein that functions as an essential component of the H\/ACA small nucleolar ribonucleoprotein (snoRNP) complex. This complex guides site-specific pseudouridylation of ribosomal RNA, a critical modification for ribosome biogenesis and proper protein synthesis.    \u003cp\u003eGAR1 interacts with other snoRNP core proteins, including DKC1, NOP10, and NHP2, to ensure stability and catalytic activity of the H\/ACA complex. Beyond its role in rRNA processing, GAR1 has been implicated in telomerase function and the regulation of RNA stability. Disruption of GAR1 activity has been associated with ribosomopathies and defects in cellular proliferation. \u003c\/p\u003e\n\u003cp\u003eUsing a high-quality GAR1 antibody enables accurate detection in applications such as western blot, immunohistochemistry, and immunofluorescence. A GAR1 antibody from\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GAR1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GAR1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GAR1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GAR1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GAR1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047325327725,"sku":"RQ8911","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_bed669c2-d983-4491-92a3-cda4e90fa141.jpg?v=1772019447","url":"https:\/\/www.ebiohippo.com\/products\/gar1-antibody-h-aca-ribonucleoprotein-complex-subunit-1-nola1-bha17136330","provider":"BioHippo","version":"1.0","type":"link"}