{"product_id":"garem1-antibody-grb2-associated-and-regulator-of-mapk-protein-1-bha17135394","title":"GAREM1 Antibody \/ GRB2-associated and regulator of MAPK protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGAREM1 Antibody \/ GRB2-associated and regulator of MAPK protein 1 is a anti-GAREM1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GAREM1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGAREM1 antibody detects GRB2-associated regulator of MAPK1 subtype 1, an adaptor protein involved in growth factor receptor signaling, particularly within the epidermal growth factor receptor (EGFR) and fibroblast growth factor receptor (FGFR) pathways. GAREM1 acts as a downstream effector of receptor tyrosine kinases and plays a role in mitogen-activated protein kinase (MAPK) signaling, cellular proliferation, and differentiation. The GAREM1 antibody is used to investigate mechanisms of growth factor signaling, cell migration, and oncogenic transformation.\u003cbr\u003e\u003cbr\u003eGAREM1 is encoded by the GAREM1 gene located on human chromosome 18p11.32. The protein is approximately 95 kilodaltons and contains multiple functional regions, including a pleckstrin homology (PH) domain, proline-rich motifs, and SH3-binding sequences that facilitate interaction with growth factor receptor-bound protein 2 (GRB2). Through these interactions, GAREM1 acts as a scaffolding adaptor that transmits extracellular growth factor signals to downstream MAPK\/ERK cascades, modulating transcriptional responses and cytoskeletal reorganization.\u003cbr\u003e\u003cbr\u003eThe GAREM1 antibody detects a 90-100 kilodalton protein on western blot and reveals cytoplasmic and perinuclear localization by immunofluorescence. Activation of EGFR or FGFR promotes tyrosine phosphorylation of GAREM1, which enhances its association with signaling molecules such as SHP2, ERK1\/2, and SOS1. Functionally, GAREM1 contributes to cell cycle progression and survival, and its overexpression has been observed in certain cancers, including hepatocellular carcinoma and glioblastoma. Depletion or inhibition of GAREM1 disrupts ERK activation, leading to reduced cell proliferation and migration.\u003cbr\u003e\u003cbr\u003eGAREM1 also influences differentiation processes by regulating cytoskeletal dynamics and cell adhesion. In neurons, GAREM1 modulates neurite outgrowth and receptor clustering, linking receptor tyrosine kinase signaling to actin remodeling. It may further act as a regulatory hub integrating MAPK and PI3K signaling pathways.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GAREM1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GAREM1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GAREM1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GAREM1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GAREM1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289545069,"sku":"FY12491","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7d2e04c1-414c-476c-8712-9e35dedbe6c3.jpg?v=1782237005","url":"https:\/\/www.ebiohippo.com\/products\/garem1-antibody-grb2-associated-and-regulator-of-mapk-protein-1-bha17135394","provider":"BioHippo","version":"1.0","type":"link"}