{"product_id":"gars1-antibody-glycyl-trna-synthetase-bha17135868","title":"GARS1 Antibody \/ Glycyl-tRNA synthetase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGARS1 Antibody \/ Glycyl-tRNA synthetase is a anti-GARS1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GARS1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGARS1 antibody detects Glycyl-tRNA synthetase, an essential enzyme that catalyzes the attachment of glycine to its corresponding tRNA during protein translation. The UniProt recommended name is Glycyl-tRNA synthetase (GARS1), a class II aminoacyl-tRNA synthetase that ensures fidelity in decoding genetic information into functional proteins. GARS1 is critical for both cytoplasmic and mitochondrial protein synthesis, as it charges tRNAGly with glycine using ATP-dependent activation.\u003cbr\u003e\u003cbr\u003eFunctionally, GARS1 antibody identifies a 739-amino-acid enzyme that performs the aminoacylation reaction in two steps: activation of glycine to form glycyl-adenylate, followed by transfer to tRNAGly. This reaction supplies aminoacyl-tRNA for translation elongation, ensuring proper incorporation of glycine into nascent polypeptides. GARS1 operates as a homodimer and belongs to the multi-synthetase complex in higher eukaryotes, coordinating translation efficiency and cellular growth. Its catalytic precision is essential for maintaining proteome integrity and translational accuracy.\u003cbr\u003e\u003cbr\u003eThe GARS1 gene is located on chromosome 7p15.3 and is ubiquitously expressed in all tissues, reflecting its fundamental role in protein biosynthesis. Mutations in GARS1 cause Charcot-Marie-Tooth disease type 2D (CMT2D) and distal spinal muscular atrophy type V, which are characterized by axonal degeneration and motor neuron dysfunction. These mutations often alter enzyme dimerization or substrate binding, impairing aminoacylation activity and disrupting neuronal protein synthesis.\u003cbr\u003e\u003cbr\u003eBeyond its canonical translation function, GARS1 exhibits moonlighting roles in signaling and neuroprotection. It can interact with neuropilin-1 and vascular endothelial growth factor (VEGF) receptors to modulate axonal guidance and angiogenesis. In response to cellular stress, GARS1 may relocate to stress granules, linking protein translation to adaptive stress responses. Overexpression or aggregation of mutant GARS1 variants contributes to neuronal toxicity and mitochondrial dysfunction, highlighting its importance in neurodegenerative disease models.\u003cbr\u003e\u003cbr\u003eGARS1 antibody is used in molecular biology and neurobiology research to examine aminoacylation, translation control, and disease-associated mutations. It is suited for immunoblotting, enzyme activity assays, and immunofluorescence studies of cytoplasmic and mitochondrial distribution. In cancer research, increased GARS1 expression has been linked to enhanced translation rates and tumor proliferation. Its detection also provides insights into translational stress responses in metabolic and neurological disorders.\u003cbr\u003e\u003cbr\u003eStructurally, GARS1 consists of a catalytic domain containing the active-site lysine responsible for ATP-dependent amino acid activation, a tRNA-binding domain, and a dimerization interface. The enzyme's activity is modulated by phosphorylation and association with other aminoacyl-tRNA synthetases within multi-enzyme assemblies.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GARS1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GARS1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GARS1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GARS1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GARS1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047308550509,"sku":"FY12966","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e58b4898-18b4-43b0-85fe-0ffbba42a4d9.jpg?v=1782237073","url":"https:\/\/www.ebiohippo.com\/products\/gars1-antibody-glycyl-trna-synthetase-bha17135868","provider":"BioHippo","version":"1.0","type":"link"}