{"product_id":"gatb-antibody-glutamyl-trna-amidotransferase-subunit-b-bha17135795","title":"GATB Antibody \/ Glutamyl-tRNA amidotransferase subunit B","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGATB Antibody \/ Glutamyl-tRNA amidotransferase subunit B is a anti-GATB Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GATB\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGATB antibody detects Glutamyl-tRNA amidotransferase subunit B, a mitochondrial enzyme subunit essential for tRNA-dependent amino acid amidation. Encoded by the GATB gene on chromosome 4q31.3, this protein forms part of the heterotrimeric GatCAB complex, which converts misacylated Glu-tRNA(Gln) and Asp-tRNA(Asn) into correctly charged Gln-tRNA(Gln) and Asn-tRNA(Asn), respectively. This transamidation reaction ensures translational accuracy and maintains proper protein synthesis in mitochondria and prokaryote-like systems.\u003cbr\u003e\u003cbr\u003eStructurally, GATB is a 471-amino-acid mitochondrial protein of approximately 52 kilodaltons that contains an amidase domain and ATP-binding sites required for the transamidation process. It associates with the catalytic subunit GATC and the tRNA recognition subunit GATA to form the functional GatCAB complex. Within mitochondria, GATB localizes to the matrix, where it couples amino acid activation with tRNA modification to sustain accurate translation of mitochondrial-encoded proteins.\u003cbr\u003e\u003cbr\u003eThe GATB antibody is widely used in mitochondrial biology, enzymology, and translation research to study aminoacyl-tRNA synthesis, protein biosynthesis fidelity, and mitochondrial genome maintenance. Western blot analysis detects a 52 kilodalton band corresponding to GATB, while immunofluorescence shows punctate mitochondrial staining that colocalizes with mitochondrial matrix markers such as HSP60. This antibody provides a dependable reagent for monitoring translational control and enzyme complex integrity in mitochondria.\u003cbr\u003e\u003cbr\u003eFunctionally, GATB ensures translational fidelity by catalyzing the amination of misacylated tRNAs, preventing incorporation of incorrect amino acids during protein synthesis. Loss or mutation of GATB disrupts mitochondrial translation and compromises respiratory chain assembly, leading to mitochondrial dysfunction, energy deficiency, and developmental disorders. Altered expression of GATB has been linked to neurodegenerative conditions and metabolic syndromes associated with impaired mitochondrial translation. The GATB antibody enables mechanistic studies of mitochondrial gene expression, enzyme complex assembly, and translational regulation under physiological and stress conditions.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GATB - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GATB - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GATB - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GATB - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GATB+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047305339245,"sku":"FY12893","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f14db326-b0ec-4bd5-8aa5-5010c69ad8d0.jpg?v=1782237047","url":"https:\/\/www.ebiohippo.com\/products\/gatb-antibody-glutamyl-trna-amidotransferase-subunit-b-bha17135795","provider":"BioHippo","version":"1.0","type":"link"}