{"product_id":"gbf1-antibody-golgi-specific-brefeldin-a-resistance-factor-1-bha17135974","title":"GBF1 Antibody \/ Golgi-specific brefeldin A-resistance factor 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGBF1 Antibody \/ Golgi-specific brefeldin A-resistance factor 1 is a anti-GBF1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Golgi.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GBF1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGBF1 antibody detects Golgi-specific brefeldin A-resistance factor 1, a guanine nucleotide exchange factor that activates ADP-ribosylation factor (ARF) GTPases controlling membrane trafficking. The UniProt recommended name is Golgi-specific brefeldin A-resistance factor 1 (GBF1). This large cytosolic protein is essential for vesicle formation, Golgi integrity, and maintenance of the secretory pathway.\u003cbr\u003e\u003cbr\u003eFunctionally, GBF1 antibody identifies a 1859-amino-acid protein that catalyzes GDP-GTP exchange on ARF1, ARF3, and related GTPases, stimulating COPI-coated vesicle assembly and Golgi-to-ER transport. GBF1 localizes to the cis-Golgi and early endosomes, where it regulates membrane curvature and vesicle budding. Brefeldin A inhibits many GEFs but not GBF1, making it a critical regulator of Golgi architecture during stress or drug treatment.\u003cbr\u003e\u003cbr\u003eThe GBF1 gene is located on chromosome 10q24.32 and is ubiquitously expressed in mammalian cells, with high abundance in secretory tissues. GBF1 coordinates with its effectors, such as BIG1 and BIG2, to sustain bidirectional membrane traffic between the endoplasmic reticulum and Golgi complex. Its activity is modulated by lipid composition, phosphorylation, and interaction with small GTPases and coatomer complexes.\u003cbr\u003e\u003cbr\u003ePathologically, GBF1 dysfunction disrupts vesicular trafficking and contributes to Golgi fragmentation, neurodegeneration, and viral replication. Several RNA viruses exploit GBF1 to remodel host membranes for replication complexes. Conversely, depletion of GBF1 can impair secretion and protein glycosylation, leading to ER stress and altered cellular homeostasis.\u003cbr\u003e\u003cbr\u003eGBF1 antibody is widely used in cell biology and virology research to study Golgi dynamics, ARF signaling, and vesicle trafficking. It is suitable for western blotting, immunofluorescence, and immunoprecipitation. \u003cbr\u003e\u003cbr\u003eStructurally, GBF1 contains a Sec7 catalytic domain responsible for nucleotide exchange, flanked by dimerization and regulatory domains that ensure specificity for ARF family substrates. Its structural organization supports dynamic localization to Golgi membranes and adaptation to cellular conditions. This antibody enables detailed study of Golgi organization, secretory pathway regulation, and virus-host interactions.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GBF1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GBF1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GBF1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GBF1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GBF1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047311106413,"sku":"FY13072","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_48acb784-cbe6-43a3-9844-5bb2ee81f136.jpg?v=1782237096","url":"https:\/\/www.ebiohippo.com\/products\/gbf1-antibody-golgi-specific-brefeldin-a-resistance-factor-1-bha17135974","provider":"BioHippo","version":"1.0","type":"link"}