{"product_id":"gch1-antibody-gtp-cyclohydrolase-1-bha17135833","title":"GCH1 Antibody \/ GTP cyclohydrolase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGCH1 Antibody \/ GTP cyclohydrolase 1 is a anti-GCH1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GCH1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGCH1 antibody detects GTP cyclohydrolase 1, the first and rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin (BH4), an essential cofactor for aromatic amino acid hydroxylases and nitric oxide synthases. The UniProt recommended name is GTP cyclohydrolase 1 (GCH1), also known as GTP-CH-I, guanosine triphosphate cyclohydrolase I, and dopa-responsive dystonia protein. GCH1 converts GTP to dihydroneopterin triphosphate, a critical step in the BH4 biosynthetic pathway, thereby controlling the availability of BH4 for dopamine, serotonin, and nitric oxide production.\u003cbr\u003e\u003cbr\u003eGCH1 antibody applications extend across neuroscience, cardiovascular research, and metabolic disorders. BH4 deficiency due to mutations in GCH1 results in disorders such as dopa-responsive dystonia (Segawa's disease) and hyperphenylalaninemia. GCH1 expression is tightly regulated by inflammatory signals, oxidative stress, and cytokines such as TNF-alpha and IFN-gamma. In endothelial cells, GCH1 controls endothelial nitric oxide synthase (eNOS) coupling and NO production, influencing vascular tone, blood pressure, and oxidative balance. Dysregulation or polymorphisms of GCH1 have been associated with hypertension, diabetes, and neuropathic pain syndromes.\u003cbr\u003e\u003cbr\u003eAt the cellular level, GCH1 antibody helps characterize tissue-specific expression patterns and study enzyme regulation in neuronal, hepatic, and vascular tissues. The GCH1 enzyme functions as a homodecamer, with allosteric regulation mediated by the feedback regulatory protein GFRP (GTP cyclohydrolase I feedback regulatory protein). Together, GCH1 and GFRP form a complex controlling BH4 biosynthesis according to phenylalanine and BH4 levels. GCH1 is encoded by the GCH1 gene located on chromosome 14q22.1-q22.2, producing a 250-amino acid cytosolic protein. Mutations include both autosomal dominant and recessive variants leading to enzymatic dysfunction and neurotransmitter deficiency syndromes.\u003cbr\u003e\u003cbr\u003eBeyond metabolic function, GCH1 expression increases in response to oxidative stress and acts as a cellular protector by enhancing antioxidant capacity. Overexpression can reduce reactive oxygen species, while silencing sensitizes cells to oxidative damage.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GCH1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GCH1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GCH1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GCH1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GCH1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306879341,"sku":"FY12931","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7a56ef4f-ed70-4a7a-a09e-19f97bdab838.jpg?v=1782237071","url":"https:\/\/www.ebiohippo.com\/products\/gch1-antibody-gtp-cyclohydrolase-1-bha17135833","provider":"BioHippo","version":"1.0","type":"link"}