{"product_id":"gcnt2-antibody-beta-1-6-n-acetylglucosaminyltransferase-2-bha17136252","title":"GCNT2 Antibody \/ Beta-1,6-N-acetylglucosaminyltransferase 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGCNT2 Antibody \/ Beta-1,6-N-acetylglucosaminyltransferase 2 is a anti-GCNT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Golgi).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GCNT2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGCNT2 antibody detects Beta-1,6-N-acetylglucosaminyltransferase 2, a Golgi-localized glycosyltransferase encoded by the GCNT2 gene on chromosome 6p24.3. GCNT2 is an enzyme that catalyzes the branching of poly-N-acetyllactosamine chains on glycoproteins and glycolipids, forming the I and i blood group antigens. It belongs to the glycosyltransferase 14 family and plays an essential role in the biosynthesis of complex glycans, particularly those expressed on erythrocytes, epithelial cells, and the ocular lens. GCNT2 expression is highest in red blood cells, corneal tissue, and gastrointestinal epithelium, where it contributes to cell adhesion and recognition processes.\u003cbr\u003e\u003cbr\u003eGCNT2 functions by transferring N-acetylglucosamine residues via a beta1,6 linkage to galactose units, generating branched carbohydrate structures. These modifications affect protein stability, receptor signaling, and cell-cell interactions. The enzyme has three transcript isoforms (A, B, and C), each exhibiting tissue-specific expression patterns and promoter usage. GCNT2A is predominant in erythroid cells, while GCNT2B and GCNT2C are expressed in non-hematopoietic tissues such as the lens and gastrointestinal tract.\u003cbr\u003e\u003cbr\u003eStructurally, GCNT2 contains a conserved catalytic domain typical of glycosyltransferases, including the DXH motif required for donor sugar binding. It is anchored in the Golgi membrane by a short N-terminal transmembrane domain, positioning its catalytic site within the lumen for glycan modification. GCNT2 belongs to the GT14 family of glycosyltransferases, which also includes GCNT1 and GCNT3, enzymes responsible for branching of mucin-type O-glycans and other carbohydrate chains.\u003cbr\u003e\u003cbr\u003eFunctionally, GCNT2 is responsible for converting linear i antigens into branched I antigens during erythrocyte maturation. Loss of GCNT2 activity results in the adult i blood group phenotype, characterized by persistence of fetal-type linear glycans. In the lens, GCNT2 participates in glycoprotein processing required for lens transparency, while in epithelial tissues, it regulates mucin glycosylation that influences cell adhesion and immune defense. Known substrates of GCNT2 include glycoproteins bearing poly-N-acetyllactosamine extensions such as laminin and selectins.\u003cbr\u003e\u003cbr\u003eMutations in GCNT2 are associated with congenital cataracts and the rare adult i blood group phenotype. Reduced enzymatic activity leads to accumulation of unbranched glycan structures, affecting membrane organization and cell surface recognition. In cancer, altered GCNT2 expression has been observed in breast, colon, and gastric carcinomas, where changes in glycan branching influence cell motility and metastasis. Pathway associations include glycosphingolipid biosynthesis, protein glycosylation, and immune recognition processes.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using GCNT2 antibody shows Golgi localization in epithelial and erythroid cells. The GCNT2 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GCNT2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GCNT2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GCNT2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GCNT2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GCNT2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323033965,"sku":"FY13350","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c12e647b-71b8-483a-b586-2657bd4f4e06.jpg?v=1782237107","url":"https:\/\/www.ebiohippo.com\/products\/gcnt2-antibody-beta-1-6-n-acetylglucosaminyltransferase-2-bha17136252","provider":"BioHippo","version":"1.0","type":"link"}