{"product_id":"gcsam-antibody-fitc-conjugated-bha10515321","title":"GCSAM Antibody, FITC conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-GCSAM antibody raised in Rabbit, conjugated with FITC. It is designed to detect GCSAM protein in Human and supports researchers working in immunology contexts where consistent antibody performance is required.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human Germinal center-associated signaling and motility protein (1-178AA) — the immunizing antigen determines the epitope region; confirm epitope compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents. Use matched secondaries to avoid no-signal or cross-reactivity issues.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal format:\u003c\/strong\u003e Recognizes multiple epitopes, providing robust signal across varied preparations and species variants. Inherent lot-to-lot variability requires appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Compatible with standard Protein A\/G purification and widely supported by secondary reagents. Include an isotype-matched control at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches for specific immunoglobulin classes, reducing non-specific populations and improving signal-to-noise.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFITC conjugate:\u003c\/strong\u003e Direct fluorescence detection (Ex ~490\/Em ~525 nm). FITC is photolabile; protect samples and use antifade reagents for slides.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eGCSAM\u003c\/strong\u003e (also referred to as GCAT2, Gcet, GCET2 germinal center expressed transcript 2, GCET2 germinal centre expressed transcript 2, Gcsam, GCSAM_HUMAN, Germinal center associated lymphoma , Germinal center associated lymphoma protein, Germinal center B cell associated protein 2, Germinal center B-cell-expressed transcript 2 protein, Germinal center expressed transcript 2, Germinal center-associated lymphoma protein, Germinal center-associated signaling and motility protein, Germinal centre associated lymphoma , Germinal centre associated lymphoma protein, Germinal centre B cell associated protein 2, Germinal centre expressed transcript 2, hGAL, M17, M17 L) is a protein target studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection and experimental design. Consult UniProt, NCBI Gene, and primary literature for current annotation of GCSAM biology, including known isoforms, interactors, and disease-relevant expression patterns in immunology.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRefer to product documentation for confirmed application data and recommended conditions.\u003c\/li\u003e\n\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins or isoforms. Orthogonal validation (siRNA, KO lysate, recombinant protein) is recommended to confirm signal specificity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix (serum, plasma, lysate, homogenate) can affect performance. Pilot dilution linearity and spike-recovery experiments are recommended for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human. Extrapolation to untested species requires empirical validation given potential epitope sequence divergence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources (internal):\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio datasheet: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53239714316653,"sku":"CSB-PA854052LC01HU-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53239807181165,"sku":"CSB-PA854052LC01HU-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Cusabio_Antibody_Placeholder_3b636baa-7e99-4f6c-bcf7-f7b8cc9b49e1.jpg?v=1784926444","url":"https:\/\/www.ebiohippo.com\/products\/gcsam-antibody-fitc-conjugated-bha10515321","provider":"BioHippo","version":"1.0","type":"link"}