{"product_id":"gel-shift-single-strand-dna-binding-assay-kit-bht16300159","title":"Gel Shift Single-strand DNA Binding Assay Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Binding Assay Kit\u003c\/span\u003e\u003cp\u003eSingle-strand DNA-binding protein (SSB) binds single-stranded DNA, or single-stranded regions of double-stranded DNA, during DNA replication, recombination and repair; for example, DNA helicase unwinding creates single-strand DNA. E. coli SSB forms a homotetramer of 19 kDa subunits.\u003c\/p\u003e\n\u003ch2\u003eKit vs Kit Plus\u003c\/h2\u003e\n\u003cp\u003eThe only difference is that GSSB100KE (Kit Plus) also includes 150 µl of 2 mg\/ml E. coli SSB protein; GSSB100K contains the buffer, ssDNA and loading buffer only, for use with your own SSB protein.\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eKit (SSB protein not included), 100 assays (GSSB100K)\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Plus (SSB protein included), 100 assays (GSSB100KE)\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eKit (SSB protein not included), 100 assays (GSSB100K)\u003c\/th\u003e\n\u003cth\u003eKit Plus (SSB protein included), 100 assays (GSSB100KE)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x Buffer\u003c\/td\u003e\n\u003ctd\u003e150 µl\u003c\/td\u003e\n\u003ctd\u003e150 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x ssDNA (45-mer single-strand DNA)\u003c\/td\u003e\n\u003ctd\u003e150 µl\u003c\/td\u003e\n\u003ctd\u003e150 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e4 x Gel loading buffer\u003c\/td\u003e\n\u003ctd\u003e100 µl\u003c\/td\u003e\n\u003ctd\u003e100 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE. coli SSB protein (2 mg\/ml)\u003c\/td\u003e\n\u003ctd\u003eNot included\u003c\/td\u003e\n\u003ctd\u003e150 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eSSB is mixed with a 45-mer single-strand DNA and run on a conventional acrylamide gel. SSB-bound DNA migrates more slowly than free DNA, so binding activity shows as a band shift.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Target\u003c\/th\u003e\n\u003ctd\u003eSSB single-strand DNA binding\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Type\u003c\/th\u003e\n\u003ctd\u003eSingle-strand DNA-binding protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eBinding Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eGel Electrophoresis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eThe manufacturer's PDF describes the assay and kit contents; please contact us for the full procedure.\u003c\/p\u003e\n\u003cul\u003e\u003cli\u003eGel Shift Single-strand DNA Binding Assay (PDF, see Documents): Covers catalog # GSSB100K, GSSB100KE.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The Gel Shift Single-strand DNA Binding Assay Kit is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Kit (SSB protein not included)","offer_id":67794881839469,"sku":"GSSB100K","price":207.0,"currency_code":"USD","in_stock":true},{"title":"Kit Plus (SSB protein included)","offer_id":67794884788589,"sku":"GSSB100KE","price":612.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/GSSB100KE_fig.jpg?v=1792791971","url":"https:\/\/www.ebiohippo.com\/products\/gel-shift-single-strand-dna-binding-assay-kit-bht16300159","provider":"BioHippo","version":"1.0","type":"link"}