{"product_id":"genjet-in-vitro-dna-transfection-reagent-bht2200003","title":"GenJet™ In Vitro DNA Transfection Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eA unique chemistry sits behind GenJet™ DNA In Vitro Transfection Reagent: cationic lipids are covalently cross-linked to polymers, and SignaGen credits that design for exceptional performance and clearly distinguishable advantages next to conventional transfection reagents. The product is powerful and reliable, reaching high transfection efficiency while cytotoxicity stays minimal and is frequently undetectable. Gene delivery has been demonstrated across many established lines and primary cells — HEK293, 293T, 293E, CHO, COS-1, HeLa and NIH\/3T3 among them, plus the insect lines Sf9 and Sf21 and numerous further eukaryotic cell types.\u003c\/p\u003e\n\u003cp\u003eEach 1.0 mL vial of GenJet™ Reagent handles roughly 666 transfections at 24-well scale, or 333 at 6-well scale.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. When stored under recommended conditions, the product is stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSupports exceptionally high viral titers\u003c\/li\u003e\n\u003cli\u003eHighly effective with large DNA constructs, up to 56 kb\u003c\/li\u003e\n\u003cli\u003eWorks equally well for single transfection or co-transfection of multiple DNA constructs\u003c\/li\u003e\n\u003cli\u003eEnables exceptionally high levels of recombinant protein expression\u003c\/li\u003e\n\u003cli\u003eWell suited to hard-to-transfect cell types\u003c\/li\u003e\n\u003cli\u003eCost-effective transfection option\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-LongDNA.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – 14 kb GFP-tagged DNA transfection in HEK293 cells vs FuGene™ 6\" loading=\"lazy\"\u003e\u003cfigcaption\u003eExceptional transfection efficiency of extremely long DNA by GenJet™ DNA In Vitro Transfection Reagent. HEK293 cells were transfected with GFP-tagged DNA (total 14 kb) by GenJet™ (upper panel) and FuGene™ 6 (lower panel), respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase (left panel) and FITC (right panel) imaging 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-Lentivirus.gif?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – lentivirus titers from 293F cells vs Lipofectamine 2000 by FACS\" loading=\"lazy\"\u003e\u003cfigcaption\u003eExceptionally high titers of lentivirus were generated from 293F cells. Three DNAs co-transfected with GenJet™ In Vitro DNA Transfection Reagent (left panel) in comparison of LipoFectamine 2000™ (L2K, right panel). 100 microliter virus supernatant from 293F cells was used to counter-infect 293F cells, which were then passed through FACS. The numbers in the upper right corner indicate the percentage of transduced cells. The titers of lentivirus generated with GenJet™ and L2K were quantified to be 7.9×106 and 3.1×106 tu\/mL, respectively\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet3.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – GFP transfection in primary murine skin fibroblasts, DIC and FITC imaging\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-FuGene1.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – primary murine skin fibroblasts transfected with FuGene™ HD for comparison\" loading=\"lazy\"\u003e\u003cfigcaption\u003ePrimary Murine Skin Fibroblast transfected with pEGFP-C1 plasmid using GenJet™ In Vitro DNA Transfection Reagent (upper panel) and the most popular brand product FuGene™ HD (lower panel). The cells were visualized by a Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-KE.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – GFP transfection in primary murine keratinocytes, DIC and FITC imaging\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-FuGene-KE.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – primary murine keratinocytes transfected with FuGene™ HD for comparison\" loading=\"lazy\"\u003e\u003cfigcaption\u003ePrimary Murine Keratinocytes transfected with pEGFP-C1 plasmid using GenJet™ In Vitro DNA Transfection Reagent (upper panel) and one of the most popular brand products, FuGene™ HD (lower panel). The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-Toxicity1.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent – cytotoxicity vs L2K™ in primary murine skin fibroblasts, DIC imaging\" loading=\"lazy\"\u003e\u003cfigcaption\u003eComparison of cytotoxicity of GenJet™ DNA In Vitro Transfection Reagent with L2K™ on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents\/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium, followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch3\u003eTransfection Efficiency by Cell Line\u003c\/h3\u003e\n\u003ctable class=\"bhp-spec-table bhp-data-table\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eCell Lines\u003c\/th\u003e\n\u003cth\u003eTransfection Efficiency (% GFP)\u003c\/th\u003e\n\u003cth\u003eCell Lines\u003c\/th\u003e\n\u003cth\u003eTransfection Efficiency (% GFP)\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e3LL\u003c\/td\u003e\n\u003ctd\u003e51%\u003c\/td\u003e\n\u003ctd\u003eK562\u003c\/td\u003e\n\u003ctd\u003e22%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eB16-F10\u003c\/td\u003e\n\u003ctd\u003e72%\u003c\/td\u003e\n\u003ctd\u003eL929\u003c\/td\u003e\n\u003ctd\u003e52%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBAEC\u003c\/td\u003e\n\u003ctd\u003e52%\u003c\/td\u003e\n\u003ctd\u003eMCF-7\u003c\/td\u003e\n\u003ctd\u003e51%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBHK-21\u003c\/td\u003e\n\u003ctd\u003e86%\u003c\/td\u003e\n\u003ctd\u003eMDCK\u003c\/td\u003e\n\u003ctd\u003e52%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCa Ski\u003c\/td\u003e\n\u003ctd\u003e78%\u003c\/td\u003e\n\u003ctd\u003eNeuro2A\u003c\/td\u003e\n\u003ctd\u003e72%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCaCo-2\u003c\/td\u003e\n\u003ctd\u003e32%\u003c\/td\u003e\n\u003ctd\u003eNIH 3T3\u003c\/td\u003e\n\u003ctd\u003e88%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCHO\u003c\/td\u003e\n\u003ctd\u003e83%\u003c\/td\u003e\n\u003ctd\u003ePC12\u003c\/td\u003e\n\u003ctd\u003e34%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHCS-2\/8\u003c\/td\u003e\n\u003ctd\u003e42%\u003c\/td\u003e\n\u003ctd\u003eSH-SY5Y\u003c\/td\u003e\n\u003ctd\u003e14%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHEK-293\u003c\/td\u003e\n\u003ctd\u003e80-90%\u003c\/td\u003e\n\u003ctd\u003eSiHa\u003c\/td\u003e\n\u003ctd\u003e62%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeLa\u003c\/td\u003e\n\u003ctd\u003e81%\u003c\/td\u003e\n\u003ctd\u003eSKOV3\u003c\/td\u003e\n\u003ctd\u003e52%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHLMEC\u003c\/td\u003e\n\u003ctd\u003e50-65%\u003c\/td\u003e\n\u003ctd\u003eHUVEC\u003c\/td\u003e\n\u003ctd\u003e51%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eH-MVEC\u003c\/td\u003e\n\u003ctd\u003e49%\u003c\/td\u003e\n\u003ctd\u003eIGROV1\u003c\/td\u003e\n\u003ctd\u003e21%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuh-7D12\u003c\/td\u003e\n\u003ctd\u003e22%\u003c\/td\u003e\n\u003ctd\u003eJurkat\u003c\/td\u003e\n\u003ctd\u003e5%-15%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eATT20\u003c\/td\u003e\n\u003ctd\u003e38%\u003c\/td\u003e\n\u003ctd\u003e6CSFMEo\u003c\/td\u003e\n\u003ctd\u003e73%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSK-N-SH\u003c\/td\u003e\n\u003ctd\u003e20%\u003c\/td\u003e\n\u003ctd\u003eWEHI 231\u003c\/td\u003e\n\u003ctd\u003e25%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMcArdle 7777\u003c\/td\u003e\n\u003ctd\u003e60%\u003c\/td\u003e\n\u003ctd\u003eSAOS-2\u003c\/td\u003e\n\u003ctd\u003e52%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHep3D\u003c\/td\u003e\n\u003ctd\u003e78%\u003c\/td\u003e\n\u003ctd\u003eSN56\u003c\/td\u003e\n\u003ctd\u003e69%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSHEP\u003c\/td\u003e\n\u003ctd\u003e71%\u003c\/td\u003e\n\u003ctd\u003eMC3T3-E1\u003c\/td\u003e\n\u003ctd\u003e70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMDA-MB-231\u003c\/td\u003e\n\u003ctd\u003e38%\u003c\/td\u003e\n\u003ctd\u003eBT474\u003c\/td\u003e\n\u003ctd\u003e46%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3T3-442A\u003c\/td\u003e\n\u003ctd\u003e10%\u003c\/td\u003e\n\u003ctd\u003eC2C12\u003c\/td\u003e\n\u003ctd\u003e46%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCOS-7\u003c\/td\u003e\n\u003ctd\u003e60-70%\u003c\/td\u003e\n\u003ctd\u003ePrimary melanocyte\u003c\/td\u003e\n\u003ctd\u003e46%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCV-1\u003c\/td\u003e\n\u003ctd\u003e53%\u003c\/td\u003e\n\u003ctd\u003eHela-S3\u003c\/td\u003e\n\u003ctd\u003e78%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eD 407\u003c\/td\u003e\n\u003ctd\u003e62%\u003c\/td\u003e\n\u003ctd\u003eA549\u003c\/td\u003e\n\u003ctd\u003e78%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDHD Pro.b\u003c\/td\u003e\n\u003ctd\u003e51%\u003c\/td\u003e\n\u003ctd\u003eHep G2\u003c\/td\u003e\n\u003ctd\u003e58%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLS180\u003c\/td\u003e\n\u003ctd\u003e46%\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEfficient DNA delivery into mammalian and insect cells\u003c\/li\u003e\n\u003cli\u003eDramatic enhancement of viral infection, up to 200-fold, including replication-deficient adenovirus, in hard-to-transfect cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899906925,"sku":"SL100488-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901512557,"sku":"SL100488-0P5ML","price":88.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901545325,"sku":"SL100488-1P0ML","price":166.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901578093,"sku":"SL100488-1P0MLX5","price":788.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-LongDNA_b817c0d2-cf9c-44af-8652-3445b6ba166c.jpg?v=1790175948","url":"https:\/\/www.ebiohippo.com\/products\/genjet-in-vitro-dna-transfection-reagent-bht2200003","provider":"BioHippo","version":"1.0","type":"link"}