{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-hepg2-cells-bht2200017","title":"GenJet™ In Vitro DNA Transfection Reagent for HepG2 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eHepG2, a well-characterized human hepatocellular carcinoma line, was taken from liver tissue of a 15-year-old male donor whose hepatocellular carcinoma was differentiated. Epithelial morphology and the retention of many liver-specific functions have pushed the line to the front rank of in vitro models for hepatic metabolism, gene regulation, drug discovery, toxicology, viral infection, and recombinant protein expression. Plasma proteins synthesized and secreted by HepG2 cells include albumin, transferrin, α1-antitrypsin, α2-macroglobulin, and plasminogen, and the cells answer a variety of hormonal and metabolic stimuli.\u003c\/p\u003e\n\u003cp\u003eRobust growth characteristics plus adaptability to large-scale culture systems have widened their use further across biomedical and pharmaceutical research. GenJet™ DNA In Vitro Transfection Reagent for HepG2 Cells comes tuned to the line, delivering plasmid DNA into HepG2 cells efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eHepG2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under the recommended storage conditions, the product remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ Reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates, depending on the culture format and amount of DNA used.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-HepG2.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for HepG2 Cells – HepG2 GFP transfection vs L2K and Amaxa®; serum\/antibiotic effect\" loading=\"lazy\"\u003e\u003cfigcaption\u003eGenJet™ Reagent is specifically optimized for HepG2 cells (ATCC® HB-8065™) and demonstrates superior transfection performance compared with Lipofectamine™ 2000 (L2K) and the Amaxa® electroporation system. HepG2 cells were cultured according to ATCC-recommended conditions on collagen type I–coated culture dishes and transfected with the GFP reporter plasmid pEGFP-N3. Transfection efficiency was evaluated 48 hours post-transfection. Right Panel: Comparison of transfection efficiencies achieved with GenJet™, Lipofectamine™ 2000 (L2K), and the Amaxa® electroporation system in HepG2 cells. A 4.7-kb GFP expression plasmid (pEGFP-N3) was delivered into HepG2 cells cultured on collagen-coated dishes according to each manufacturer’s recommended protocol. Transfection efficiency, expressed as the percentage of GFP-positive cells, and GFP fluorescence intensity were quantified by flow cytometry (FACS) 48 hours post-transfection. Left Panel: Effect of serum and antibiotics on GenJet™-mediated transfection in HepG2 cells. Cells cultured on collagen-coated dishes were transfected under three different conditions: (1) serum- and antibiotic-free medium; (2) medium containing 10% serum and antibiotics, followed by medium replacement 5 hours post-transfection; and (3) medium containing 10% serum and antibiotics without medium replacement following transfection. The presence of serum and antibiotics was found to enhance GenJet™-mediated gene delivery and transgene expression in HepG2 cells.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-HepG2-GFP-betaGal.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for HepG2 Cells – GFP and β-galactosidase expression in HepG2 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eRepresentative images demonstrating the transfection performance of the pre-optimized GenJet™ DNA Transfection Reagent in HepG2 cells (ATCC® HB-8065™). HepG2 cells were cultured under ATCC-recommended conditions on collagen type I–coated culture dishes and transfected with either the GFP reporter plasmid pEGFP-N3 (4.7 kb; right panel) or the β-galactosidase expression plasmid pSV-β-galactosidase (6.9 kb; left panel). Transgene expression was evaluated 48 hours post-transfection by confocal fluorescence microscopy using a Zeiss LSM 510 system for GFP detection and by β-galactosidase staining for assessment of β-galactosidase expression. The results demonstrate efficient delivery and expression of both reporter genes in HepG2 cells.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900136301,"sku":"SL100489-HEPG2-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042902888813,"sku":"SL100489-HEPG2-0P5ML","price":99.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042902921581,"sku":"SL100489-HEPG2-1P0ML","price":189.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042902954349,"sku":"SL100489-HEPG2-1P0MLX5","price":897.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-HepG2_75264513-94f9-4382-b9bc-8039793d2417.jpg?v=1790175947","url":"https:\/\/www.ebiohippo.com\/products\/genjet-in-vitro-dna-transfection-reagent-for-hepg2-cells-bht2200017","provider":"BioHippo","version":"1.0","type":"link"}