{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-huh-7-cells-bht2200018","title":"GenJet™ In Vitro DNA Transfection Reagent for HuH-7 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eHuH-7, a human hepatocellular carcinoma line, turns up often in liver biology, virology, and gene expression studies. Transfecting it is generally harder than transfecting HEK293-derived cells; proprietary peptide-conjugation technology built into GenJet™ reagent addresses that, driving plasmid DNA in at high efficiency and reaching as much as 70% GFP-positive cells under optimized conditions. GenJet™ DNA In Vitro Transfection Reagent for HuH-7 Cells therefore ships pre-tuned for transfection of human HuH-7 cells.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eHuH-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 150–300 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-HUH7.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for HuH-7 Cells – GFP transfection vs L2K and FuGENE® HD; serum\/antibiotic effect\" loading=\"lazy\"\u003e\u003cfigcaption\u003eGenJet™ reagent is specifically optimized for HuH-7 cells and demonstrates superior transfection performance compared with Lipofectamine™ 2000 (L2K) and FuGENE® HD. HuH-7 cells were cultured according to recommended conditions and transfected with the GFP expression plasmid pEGFP-N3 using GenJet™ reagent. Transfection efficiency was evaluated 48 hours post-transfection. Right Panel: Comparison of transfection efficiency among GenJet™, Lipofectamine™ 2000 (L2K), and FuGENE® HD in HuH-7 cells. Cells were transfected with pEGFP-N3 according to each manufacturer’s recommended protocol. The percentage of GFP-positive cells and relative fluorescence intensity were quantified by flow cytometry (FACS) 48 hours post-transfection. Left Panel: The presence of serum and antibiotics enhances GenJet™-mediated transfection efficiency in HuH-7 cells. HuH-7 cells cultured on collagen-coated dishes were transfected under three different conditions: (1) serum- and antibiotic-free medium; (2) medium containing 10% serum and antibiotics, with medium replacement 5 hours post-transfection; and (3) medium containing 10% serum and antibiotics without medium replacement. Transfection efficiency was assessed 48 hours post-transfection.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-HUH.jpg?v=1790173499\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for HuH-7 Cells – GFP transfection in HuH-7 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA representative fluorescence microscopy image demonstrating the transfection efficiency of the pre-optimized GenJet™ Reagent in HuH-7 cells. HuH-7 cells were cultured under recommended growth conditions on collagen type I–coated culture dishes and transfected with the GFP reporter plasmid pEGFP-N3 using GenJet™ Reagent. Transgene expression was assessed 24 hours post-transfection by fluorescence imaging with a Nikon Eclipse 2000 microscope. GFP fluorescence served as an indicator of plasmid DNA delivery and gene expression efficiency.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899972461,"sku":"SL100489-HUH-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901905773,"sku":"SL100489-HUH-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901938541,"sku":"SL100489-HUH-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901971309,"sku":"SL100489-HUH-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-HUH7_e8132042-86e0-41e7-9924-01f6a6c50a60.jpg?v=1790175947","url":"https:\/\/www.ebiohippo.com\/products\/genjet-in-vitro-dna-transfection-reagent-for-huh-7-cells-bht2200018","provider":"BioHippo","version":"1.0","type":"link"}