{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-mda-mb231-cells-bht2200023","title":"GenJet™ In Vitro DNA Transfection Reagent for MDA-MB231 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eMDA-MB-231 traces back to the pleural effusion of a 51-year-old female patient whose breast cancer had metastasized; the resulting line is classed as a human breast adenocarcinoma. Under the microscope the cells look epithelial-like and spindle-shaped, and researchers turn to them routinely as an in vitro stand-in for aggressive, highly invasive triple-negative breast cancer (TNBC). What defines the line is vigorous migration and invasion, a high metastatic potential, and growth that does not depend on anchorage.\u003c\/p\u003e\n\u003cp\u003eThose same traits make it a valuable system for probing tumor progression, epithelial–mesenchymal transition (EMT), cancer cell invasion, metastasis, and the evaluation of novel therapeutic strategies. Rapid proliferation and the line's unique biological characteristics do mean transfection conditions frequently have to be tuned so that gene delivery stays efficient and the cells stay viable. GenJet™ DNA In Vitro Transfection Reagent for MDA-MB-231 Cells ships pre-optimized for exactly that, moving plasmid DNA into MDA-MB-231 cells efficiently.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eMDA-MB231\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. When stored under the recommended conditions, GenJet™ reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300 transfections in 24-well plates or 150 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-MB231.jpg?v=1790173498\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for MDA-MB231 Cells – GFP transfection in MDA-MB-231 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA representative image demonstrating the transfection efficiency of GenJet™ Transfection Reagent in MDA-MB-231 cells. MDA-MB-231 cells were cultured under ATCC-recommended growth conditions and transfected with the GFP reporter plasmid pEGFP-N3 using GenJet™ reagent. Transfection efficiency was assessed 24 hours post-transfection by evaluating GFP expression using fluorescence microscopy.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899120493,"sku":"SL100489-MB231-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042904822125,"sku":"SL100489-MB231-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042904854893,"sku":"SL100489-MB231-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042904887661,"sku":"SL100489-MB231-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-MB231_d276963c-9f82-4a8a-aa52-14463f07d3ad.jpg?v=1790175946","url":"https:\/\/www.ebiohippo.com\/products\/genjet-in-vitro-dna-transfection-reagent-for-mda-mb231-cells-bht2200023","provider":"BioHippo","version":"1.0","type":"link"}