{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-mefs-bht2200027","title":"GenJet™ In Vitro DNA Transfection Reagent for MEFs","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eFeeder layers that support the growth and maintenance of undifferentiated embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are commonly built from mouse embryonic fibroblasts (MEFs). Isolated from mouse embryos, these primary fibroblasts are normally put to work at early passage numbers, and before serving as feeders they are usually arrested by irradiation or by mitomycin C treatment so that proliferation stops while their supportive functions carry on. Primary cells of this kind have a reputation for being hard to transfect.\u003c\/p\u003e\n\u003cp\u003eGenJet™ DNA In Vitro Transfection Reagent for Mouse Embryonic Fibroblasts (MEFs) arrives pre-optimized and conditioned for plasmid DNA delivery into MEFs, and SignaGen credits its proprietary transfection chemistry plus an optimized protocol with taking the reagent as high as 85% GFP-positive cells under optimized conditions.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eMEFs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. Under recommended storage conditions, the product is stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300 transfections in 24-well plates or 150 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet_MEF.jpg?v=1790173499\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for MEFs – GFP transfection in mouse embryonic fibroblasts (MEFs)\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA representative image demonstrating the transfection efficiency of GenJet™ Transfection Reagent in Mouse Embryonic Fibroblasts (MEFs). MEFs were cultured under recommended growth conditions and transfected with the GFP reporter plasmid pEGFP-N3 using GenJet™ reagent. Transfection efficiency was evaluated 24 hours post-transfection by assessing GFP expression using fluorescence microscopy.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899284333,"sku":"SL100489-MEF-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042903183725,"sku":"SL100489-MEF-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042903216493,"sku":"SL100489-MEF-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042903249261,"sku":"SL100489-MEF-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet_MEF_c18eda95-4180-4dcb-93d2-f8ccc8498f17.jpg?v=1790175947","url":"https:\/\/www.ebiohippo.com\/products\/genjet-in-vitro-dna-transfection-reagent-for-mefs-bht2200027","provider":"BioHippo","version":"1.0","type":"link"}