{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-neuro-2a-cells-bht2200028","title":"GenJet™ In Vitro DNA Transfection Reagent for Neuro-2A Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eNeuro2A (N2A), a mouse neuroblastoma line, is called on again and again as an in vitro model for neuronal differentiation, neurodevelopment, signal transduction, gene regulation, and neurodegenerative disorders. Put it in the right culture conditions and it will extend neurites and take on neuron-like characteristics — one reason it stays among the most commonly used neuronal cell models in biomedical research. GenJet™ DNA In Vitro Transfection Reagent for Neuro2A Cells arrives with conditions already tuned for efficient plasmid DNA delivery into these cells.\u003c\/p\u003e\n\u003cp\u003eThe protocol set out below was worked up to push efficiency to its maximum in Neuro2A cells while viability stays excellent. A single milliliter of GenJet™ reagent stretches to roughly 300–600 transfections in 24-well plates, or 150–300 in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eNeuro-2A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 150–300 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-N2A.jpg?v=1790173498\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for Neuro-2A Cells – GFP transfection in Neuro2A cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA Representative Image Demonstrating the Transfection Efficiency of GenJet™ Reagent in Neuro2A Cells. Neuro2A cells were cultured according to ATCC-recommended growth conditions and transfected with the GFP expression plasmid pEGFP-N3 using GenJet™ reagent. GFP expression was evaluated 36 hours post-transfection using a Nikon Eclipse fluorescence microscope. Differential interference contrast (DIC) imaging is shown in the left panel, and FITC fluorescence imaging is shown in the right panel.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900627821,"sku":"SL100489-N2A-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042903380333,"sku":"SL100489-N2A-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042903413101,"sku":"SL100489-N2A-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042903445869,"sku":"SL100489-N2A-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-N2A_c9f2dbae-3560-41ed-8920-afea58c4df4f.jpg?v=1790175946","url":"https:\/\/www.ebiohippo.com\/products\/genjet-in-vitro-dna-transfection-reagent-for-neuro-2a-cells-bht2200028","provider":"BioHippo","version":"1.0","type":"link"}