{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-pc-3-cells-bht2200031","title":"GenJet™ In Vitro DNA Transfection Reagent for PC-3 Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003ePC-3, a human prostate adenocarcinoma line taken from a bone metastasis, is a common choice for studies of prostate cancer progression, metastasis, signal transduction, and therapeutic development. Transfecting these cells efficiently is possible, but they often react badly to the cytotoxicity that comes with conventional lipid-based transfection reagents — viability drops and experimental outcomes suffer. GenJet™ DNA In Vitro Transfection Reagent for PC-3 Cells is tuned in advance for efficient DNA transfection of the line; SignaGen attributes to its proprietary formulation and optimization the pairing of highly efficient plasmid DNA delivery with minimal transfection-associated cytotoxicity.\u003c\/p\u003e\n\u003cp\u003eUp to 60% GFP-positive cells is attainable under optimized conditions, with viability staying excellent. The protocol below was refined to reach maximal efficiency in PC-3 cells. One milliliter of GenJet™ reagent stretches to roughly 300–600 transfections in 24-well plates, or 50–100 in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003ePC-3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 50–100 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899841389,"sku":"SL100489-PC3-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042903970157,"sku":"SL100489-PC3-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042904002925,"sku":"SL100489-PC3-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042904035693,"sku":"SL100489-PC3-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/genjet-in-vitro-dna-transfection-reagent-for-pc-3-cells-bht2200031","provider":"BioHippo","version":"1.0","type":"link"}