{"product_id":"genjet-in-vitro-dna-transfection-reagent-for-u2os-cells-bht2200030","title":"GenJet™ In Vitro DNA Transfection Reagent for U2OS Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eU2OS began with tibial tumor tissue taken from a 15-year-old female patient; the human osteosarcoma line that resulted now ranks among the most heavily used cell models in cancer biology and molecular genetics. The cells attach and adopt an epithelial-like shape, and they turn up throughout work on cell cycle regulation, DNA damage response, genome stability, signal transduction, gene expression, and cancer therapeutics. p53 function in the line remains wild-type, which is what makes it so useful for digging into tumor suppression mechanisms and cellular stress responses. GenJet™ DNA In Vitro Transfection Reagent for U2OS Cells arrives with its conditions already tuned, so DNA transfection of U2OS cells runs efficiently; the protocol below was refined to reach maximal efficiency in these cells while viability stays excellent.\u003c\/p\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent stretches to roughly 300–600 transfections in 24-well plates, or 150–300 in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eU2OS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C. When maintained under recommended storage conditions, the reagent remains stable for at least 24 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cp\u003eOne milliliter of GenJet™ reagent is sufficient for approximately 300–600 transfections in 24-well plates or 150–300 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-OS_png.webp?v=1790173498\" alt=\"GenJet™ In Vitro DNA Transfection Reagent for U2OS Cells – GFP transfection in U2OS cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA Representative Image Demonstrating the Transfection Efficiency of GenJet™ Reagent in U2OS Cells. U2OS cells were cultured according to ATCC-recommended growth conditions and transfected with the GFP expression plasmid pEGFP-N3 using GenJet™ reagent. Transfection efficiency was evaluated 48 hours post-transfection by monitoring GFP expression.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899939693,"sku":"SL100489-OS-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042904658285,"sku":"SL100489-OS-0P5ML","price":98.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042904691053,"sku":"SL100489-OS-1P0ML","price":189.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042904723821,"sku":"SL100489-OS-1P0MLX5","price":897.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-OS_png_40bb99ad-9172-4776-a203-ced43d374250.webp?v=1790175947","url":"https:\/\/www.ebiohippo.com\/products\/genjet-in-vitro-dna-transfection-reagent-for-u2os-cells-bht2200030","provider":"BioHippo","version":"1.0","type":"link"}