{"product_id":"genjet-in-vitro-dna-transfection-reagent-ver-ii-bht2200004","title":"GenJet™ In Vitro DNA Transfection Reagent (Ver. II)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eGenJet™ In Vitro DNA Transfection Reagent (Ver. II) upgrades the original GenJet™ In Vitro DNA Transfection Reagent through a reformulated chemistry. That chemistry liberates DNA-condensing groups at 100%, where the earlier version reached 80%, and transfection efficiency climbs 3–4 fold as a result, with cell viability left intact.\u003c\/p\u003e\n\u003cp\u003eCoverage per 1.0 mL vial of GenJet™ (Ver. II) Reagent runs to about 667 transfections at 24-well scale, or 333 at 6-well scale.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. When stored under recommended conditions, the product remains stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e3–4 fold higher transfection efficiency, depending on cell type\u003c\/li\u003e\n\u003cli\u003ePreferred option for hard-to-transfect cells\u003c\/li\u003e\n\u003cli\u003eImproved transfection efficiency with serum and antibiotics present, for most cell types\u003c\/li\u003e\n\u003cli\u003eSupports exceptionally high levels of recombinant protein expression\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJetVII-1.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent (Ver. II) – luciferase efficiency and MTT toxicity vs original GenJet™\" loading=\"lazy\"\u003e\u003cfigcaption\u003eComparison of transfection efficiency (left panel) and toxicity (right panel) GenJet™ Ver. II with the old version of GenJet™ on commonly used mammalian cells. phRL-CMV encoding Renilla Luciferase was introduced into cells in the presence of serum and antibiotics. The Renilla Luciferase activity was determined with the Renilla Luciferase Assay System (Promega). For cytotoxicity, the MTT assay was used to determine cell viability, with the untransfected control set at 100%.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJet-HartoCell2.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent (Ver. II) – nuclear GFP in primary rat aortic smooth muscle cells vs L2K\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison of the transfection efficiency of GenJet™ (Ver. II) vs. Lipofectamine 2000 on primary rat aortic smooth muscle cells. Primary rat aortic smooth muscle cells were prepared and transfected with a nuclear-targeted GFP cDNA by GenJet™ (right panel) and Lipofectamine 2000 (L2K, left panel), respectively. The transfection efficiency was checked under a Nikon Eclipse 2000 fluorescence microscope 48 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJetVII-2.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent (Ver. II) – GFP transfection vs original GenJet™ in 293T and HeLa cells\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJetVII-3II.jpg?v=1790173423\" alt=\"GenJet™ In Vitro DNA Transfection Reagent (Ver. II) – GFP transfection vs original GenJet™ in 293T and HeLa cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eUpper Panel: Transfection efficiency comparison of GenJet™ Ver. II with the old version of GenJet™ on 293T cells. pEGFP-N3 was transfected into 293T cells at 90% confluency in the presence of serum and antibiotics. GFP fluorescence was detected with Nikon Eclipse 2000 microscopy 48 hours post-transfection Lower panel: Transfection efficiency comparison of GenJet™ Ver. II with the old version of GenJet™ on Hela cells. pEGFP-N3 was transfected into Hela cells at 90% in the presence of serum and antibiotics. GFP fluorescence was detected with Nikon Eclipse 2000 microscopy 48 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899579245,"sku":"SL100489-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901709165,"sku":"SL100489-0P5ML","price":96.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901741933,"sku":"SL100489-1P0ML","price":178.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901774701,"sku":"SL100489-1P0MLX5","price":845.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenJetVII-2_3ff4b1c8-4715-4da3-82a4-dbc788b3c529.jpg?v=1790175949","url":"https:\/\/www.ebiohippo.com\/products\/genjet-in-vitro-dna-transfection-reagent-ver-ii-bht2200004","provider":"BioHippo","version":"1.0","type":"link"}