{"product_id":"genmute-sirna-transfection-reagent-bht2200038","title":"GenMute™ siRNA Transfection Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eA novel biodegradable, non-liposomal tool for delivering siRNA\/miRNA and DNA, GenMute™ Reagent was put together around SignaGen's proprietary pH Dependent Conformational Change (PDCC) technology, with pre-screened hydrophobic groups attached to its side chain — an approach SignaGen credits for making it a versatile and most powerful gene delivery tool in the market. Validation shows the reagent transfecting single siRNA\/miRNA, or co-transfecting DNA\/siRNA or DNA\/miRNA, effectively and reproducibly into a variety of mammalian cells.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eBoth\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C for GenMute™ Reagent and RT for GenMute™ Transfection Buffer (5x). If stored properly, the product is stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 mL, sufficient for ~2,000 reactions based on transfecting 5.0 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExcellent silencing at low concentrations, with only 1.0 nM siRNA \/ miRNA mimics\u003c\/li\u003e\n\u003cli\u003eMinimized non-specific and off-target effects in the cell\u003c\/li\u003e\n\u003cli\u003eSingle-tube reaction\u003c\/li\u003e\n\u003cli\u003eBest suited to broad mammalian cells\u003c\/li\u003e\n\u003cli\u003eCytotoxicity kept very low\u003c\/li\u003e\n\u003cli\u003eHighly affordable\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenMute_Luc.jpg?v=1790173499\" alt=\"GenMute™ siRNA Transfection Reagent – Renilla luciferase siRNA knockdown in HEK293 vs Dharmafect™ 4 and L2K\" loading=\"lazy\"\u003e\u003cfigcaption\u003eKnockdown efficacy comparison of GenMute™ Transfection Reagent (upper panel) vs. Dharmafect™ 4 siRNA Transfection Reagent (middle panel) and Lipofectamine™ 2000 (lower panel) on HEK293 cells. siRNA targeting renilla luciferase at different final concentrations ranging from 0.5 to 20 nM was co-transfected with the renilla luciferase gene (0.5 µg of pRL-CMV DNA per well) by the above three transfection reagents per manufacturer’s protocols into HEK293 cells growing on a 24-well plate. Renilla luciferase activity was determined 24h after post co-transfection with the renilla luciferase determination system (Promega). The luminescence was measured from 5.0 µl of lysate during 10s integration with a luminometer (Beckman Coulter LD 400). Luciferase activity was expressed as light units integrated over 10s (RLU) and normalized per mg of cell protein by using the BCA assay. The error bars represent the standard deviation derived from triplicate experiments. Luciferase-silencing efficiency was calculated relative to untreated cells. While GenMute™ and Dharmafect™ 4 reagents delivered significant gene silencing from 1.0 nM of renilla luciferase siRNA, lipofectamine™ 2000 gave good knockdown only after 30 nM (data not shown).\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenMute_Eg5_293.jpg?v=1790173499\" alt=\"GenMute™ siRNA Transfection Reagent – Eg5 siRNA knockdown causing cell rounding in HEK293 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eExcellent silencing of endogenously expressed KIF11 (also known as Eg5) in HEK293 cells with 0.5 µl of GenMute™ reagent and 5.0 pmol Eg5 siRNA per well of 24-well plate. KIF11 (also known as Eg5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. GenMute™ reagent effectively delivers Eg5 siRNA (final 10 nM, right panel) to HEK293 cells at only 0.5 µl per well of 24-well plate vs. negative control (final 10 nM. left panel). Compared with the negative control (left panel), the phenotype of “rounded-up” 293 cells were visualized 24 hours post transfection (right panel) with a Nikon microscope.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenMute_Lamin.jpg?v=1790173499\" alt=\"GenMute™ siRNA Transfection Reagent – lamin A\/C siRNA knockdown in HeLa cells by immunofluorescence\" loading=\"lazy\"\u003e\u003cfigcaption\u003eGenMute™ Transfection Reagent knocked down endogenous lamin A\/C gene expression in HeLa cells. A siRNA targeting lamin A\/C gene (right panel) and a sham siRNA (left panel) were introduced into Hela cells (final 1.0 nM) by GenMute™ Transfection Reagent. Lamin A\/C gene silencing was monitored 24h post-transfection by immunofluorescence. Lamin A\/C was probed with a mouse monoclonal lamin A\/C specific antibody, followed by the addition of FITC-conjugated anti-mouse antibodies. Quantitative analysis showed that lamin siRNA at 1.0 nM delivered by GenMute™ Transfection Reagent knocked down 94% endogenously expressed lamin A\/C in Hela cells.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSingle siRNA transfection, or DNA\/siRNA co-transfection, to a variety of mammalian cells\u003c\/li\u003e\n\u003cli\u003eSingle miRNA, miRNA mimics or inhibitors transfection, or DNA\/miRNA or DNA\/miRNA mimics co-transfection, to a variety of mammalian cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900562285,"sku":"SL100568-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042905379181,"sku":"SL100568-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042905411949,"sku":"SL100568-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042905444717,"sku":"SL100568-1P0MLX5","price":988.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-GenMute-Silencing-Lamin-Gene-on-HeLa-Cell_42071f2e-1e3b-4bea-bfff-2b9727b7afd6.jpg?v=1790175947","url":"https:\/\/www.ebiohippo.com\/products\/genmute-sirna-transfection-reagent-bht2200038","provider":"BioHippo","version":"1.0","type":"link"}