{"product_id":"genmute-sirna-transfection-reagent-for-neuro-2a-cells-bht2200047","title":"GenMute™ siRNA Transfection Reagent for Neuro-2A Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eNeuro-2A (N2A) cells — a mouse neuroblastoma line serving broadly as an in vitro model for neuronal differentiation, neurodevelopment, neurotoxicity, and signal transduction studies — are what the pre-optimized GenMute™ siRNA Transfection Reagent for Neuro-2A Cells addresses, delivering siRNA into them efficiently. Transfection efficiency across N2A cultures can vary when conventional reagents are used, the more so once the cells take on a neuron-like differentiated state. This formulation sets out to raise cellular uptake and endosomal release of siRNA without compromising viability or normal cellular morphology.\u003c\/p\u003e\n\u003cp\u003eOptimal gene knockdown depends on following the transfection conditions recommended for Neuro-2A cells: cell density, siRNA amount, and incubation parameters.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eNeuro-2A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell culture medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConfluency of the cell on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~50%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA concentration\u003c\/th\u003e\n\u003ctd\u003e10 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003eRenilla Luciferase 90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 20 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899251565,"sku":"SL100568-N2A-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042904068461,"sku":"SL100568-N2A-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042904101229,"sku":"SL100568-N2A-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042904133997,"sku":"SL100568-N2A-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/genmute-sirna-transfection-reagent-for-neuro-2a-cells-bht2200047","provider":"BioHippo","version":"1.0","type":"link"}