{"product_id":"genmute-sirna-transfection-reagent-for-primary-macrophages-bht2200048","title":"GenMute™ siRNA Transfection Reagent for Primary Macrophages","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003ePrimary macrophages take up siRNA efficiently with GenMute™ siRNA Transfection Reagent for PMG, optimized for exactly that purpose. Circulating monocytes give rise to these tissue-resident white blood cells, whose roles sit at the center of innate as well as adaptive immunity. Their work as professional phagocytes involves engulfing and breaking down foreign particles, pathogens, and cellular debris, alongside secretion of the cytokines that steer immune responses.\u003c\/p\u003e\n\u003cp\u003eA characteristic marker set — CD14, CD11b, F4\/80 (mouse) or EMR1 (human), Lysozyme M, MAC-1\/MAC-3, and CD68 — allows their identification through flow cytometry or immunohistochemical staining.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003ePrimary Macrophages\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell culture medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConfluency of cell on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA concentration\u003c\/th\u003e\n\u003ctd\u003e40 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003e85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 25 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003eGenMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900169069,"sku":"SL100568-PMG-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042905018733,"sku":"SL100568-PMG-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042905051501,"sku":"SL100568-PMG-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042905084269,"sku":"SL100568-PMG-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/genmute-sirna-transfection-reagent-for-primary-macrophages-bht2200048","provider":"BioHippo","version":"1.0","type":"link"}