{"product_id":"genmute-sirna-transfection-reagent-for-smooth-muscle-cells-bht2200051","title":"GenMute™ siRNA Transfection Reagent for Smooth Muscle Cells","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003ePrimary and cultured smooth muscle cells take up siRNA efficiently with GenMute™ siRNA Transfection Reagent for Smooth Muscle Cells (SMC), which comes pre-optimized for them. Residing in hollow-organ and blood vessel walls, smooth muscle cells carry essential roles in setting vascular tone, blood pressure, and organ contractility. Their low proliferation rate and their sensitivity to cytotoxic reagents nonetheless leave SMCs hard to transfect by conventional means.\u003c\/p\u003e\n\u003cp\u003eHigh uptake of siRNA together with minimal toxicity opens up gene silencing work spanning vascular biology, cardiovascular disease, airway function, and in vitro smooth muscle physiology.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eYes — Pre-Optimized for Specific Cell Type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimized Cell Line\u003c\/th\u003e\n\u003ctd\u003eSmooth Muscle Cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 24 months or longer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eOptimal Transfection Conditions\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCell culture medium\u003c\/th\u003e\n\u003ctd\u003eDMEM with 4.5 g\/L glucose, 10% FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConfluency of cell on the day of transfection\u003c\/th\u003e\n\u003ctd\u003e~60%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOptimal siRNA concentration\u003c\/th\u003e\n\u003ctd\u003e40 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDiluent for siRNA and GenMute™ Reagent\u003c\/th\u003e\n\u003ctd\u003e1 x GenMute™ Transfection Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation Time to Form GenMute™\/siRNA Complex\u003c\/th\u003e\n\u003ctd\u003e~15 min at RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximal Silencing Timeline\u003c\/th\u003e\n\u003ctd\u003e72 hrs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReporter Gene Silencing Efficiency (% )\u003c\/th\u003e\n\u003ctd\u003eRenilla Luciferase 85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900103533,"sku":"SL100568-SMC-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042905575789,"sku":"SL100568-SMC-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042905608557,"sku":"SL100568-SMC-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042905641325,"sku":"SL100568-SMC-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/genmute-sirna-transfection-reagent-for-smooth-muscle-cells-bht2200051","provider":"BioHippo","version":"1.0","type":"link"}