{"product_id":"gfra2-antibody-gdnf-family-receptor-alpha-2-bha17135798","title":"GFRA2 Antibody \/ GDNF family receptor alpha 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGFRA2 Antibody \/ GDNF family receptor alpha 2 is a anti-GFRA2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Vesicles), cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GFRA2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGFRA2 antibody detects GDNF family receptor alpha 2, a glycosylphosphatidylinositol (GPI)-anchored receptor involved in neurotrophic signaling. Encoded by the GFRA2 gene on chromosome 8p21.3, this receptor mediates cell surface binding and signal transduction of neurturin, a member of the glial cell line-derived neurotrophic factor (GDNF) family. GFRA2 functions as a co-receptor with the transmembrane tyrosine kinase RET, promoting neuronal survival, differentiation, and axonal growth in both the central and peripheral nervous systems.\u003cbr\u003e\u003cbr\u003eStructurally, GFRA2 is a 464-amino-acid protein of approximately 50 kilodaltons, containing three cysteine-rich domains (D1-D3) essential for ligand binding. It is tethered to the cell membrane through a GPI anchor and can also exist in soluble form, allowing it to modulate neurotrophic signaling in paracrine or autocrine contexts. GFRA2 expression is enriched in parasympathetic and sensory neurons, as well as cardiac and enteric ganglia, where it contributes to neural patterning and regeneration.\u003cbr\u003e\u003cbr\u003eThe GFRA2 antibody is widely used in neurobiology, regenerative medicine, and developmental neuroscience research to study trophic signaling, axonal repair, and neuronal differentiation. Western blot analysis detects a 50 kilodalton band corresponding to GFRA2, while immunofluorescence reveals strong membrane and axonal staining in neuronal cultures and ganglia. This antibody supports studies exploring neurotrophic receptor distribution and GDNF family signaling networks.\u003cbr\u003e\u003cbr\u003eFunctionally, GFRA2 mediates the effects of neurturin by recruiting RET and activating downstream MAPK and PI3K\/AKT pathways that support neuronal survival and neurite outgrowth. Dysregulation of GFRA2 signaling has been linked to neurodegenerative disorders and impaired regeneration following nerve injury. It also plays a role in cardiac innervation and development through guidance of parasympathetic neurons. The GFRA2 antibody enables detailed analysis of these pathways and helps characterize therapeutic responses in models of neural injury and neuroprotection.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GFRA2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GFRA2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GFRA2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GFRA2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GFRA2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047305437549,"sku":"FY12896","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8289a619-0cf0-4b15-9fb0-2fcf5bdbcaa9.jpg?v=1782237063","url":"https:\/\/www.ebiohippo.com\/products\/gfra2-antibody-gdnf-family-receptor-alpha-2-bha17135798","provider":"BioHippo","version":"1.0","type":"link"}