{"product_id":"gga2-antibody-golgi-localized-gamma-ear-containing-arf-binding-protein-2-bha17135829","title":"GGA2 Antibody \/ Golgi-localized, gamma ear-containing, ARF-binding protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGGA2 Antibody \/ Golgi-localized, gamma ear-containing, ARF-binding protein 2 is a anti-GGA2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GGA2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IP, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGGA2 antibody detects Golgi-associated, gamma-adaptin ear-containing, ARF-binding protein 2, a clathrin adaptor involved in vesicular trafficking between the trans-Golgi network (TGN) and endosomes. Encoded by the GGA2 gene on chromosome 16p12.3, this cytosolic adaptor belongs to the Golgi-localized, gamma-ear-containing, ARF-binding (GGA) protein family that mediates cargo selection and vesicle formation. GGA2 plays a critical role in sorting of lysosomal enzymes and membrane receptors by linking cargo proteins to clathrin-coated vesicles.\u003cbr\u003e\u003cbr\u003eStructurally, GGA2 is a 613-amino-acid cytoplasmic protein of approximately 70 kilodaltons composed of three major domains: a VHS (Vps27, Hrs, STAM) domain that recognizes sorting signals on cargo proteins, a GAT (GGA and TOM1) domain that binds ARF1-GTP, and a GAE (gamma-adaptin ear) domain that recruits accessory proteins involved in vesicle formation. These domains coordinate to promote cargo capture, clathrin coat assembly, and vesicle budding from the TGN.\u003cbr\u003e\u003cbr\u003eThe GGA2 antibody is widely used in cell biology, membrane trafficking, and protein transport research to study endosomal sorting, Golgi organization, and receptor recycling. Western blot analysis detects a 70 kilodalton band corresponding to GGA2, while immunofluorescence reveals punctate perinuclear staining consistent with Golgi localization. This antibody serves as a valuable tool for examining vesicle-mediated transport and adaptor protein function in secretory pathways.\u003cbr\u003e\u003cbr\u003eFunctionally, GGA2 interacts with mannose-6-phosphate receptors and lysosomal hydrolase precursors, mediating their trafficking to late endosomes and lysosomes. It also contributes to receptor downregulation and recycling through ARF-dependent membrane dynamics. Loss or disruption of GGA2 impairs lysosomal enzyme delivery and can cause abnormal accumulation of cargo proteins within the Golgi apparatus. The GGA2 antibody enables detailed study of Golgi-to-endosome transport, cargo recognition mechanisms, and clathrin coat assembly.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GGA2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GGA2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GGA2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GGA2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GGA2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047305961837,"sku":"FY12927","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b82451f5-a3c8-4b55-b3da-92b02a2d5f09.jpg?v=1782237051","url":"https:\/\/www.ebiohippo.com\/products\/gga2-antibody-golgi-localized-gamma-ear-containing-arf-binding-protein-2-bha17135829","provider":"BioHippo","version":"1.0","type":"link"}