{"product_id":"ggct-antibody-gamma-glutamylcyclotransferase-bha17135304","title":"GGCT Antibody \/ Gamma-glutamylcyclotransferase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGGCT Antibody \/ Gamma-glutamylcyclotransferase is a anti-GGCT Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GGCT\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe GGCT antibody targets Gamma-glutamylcyclotransferase, an enzyme encoded by the GGCT gene that participates in glutathione metabolism and cellular antioxidant defense. Gamma-glutamylcyclotransferase catalyzes the conversion of gamma-glutamyl amino acids to 5-oxoproline and free amino acids, playing a vital role in the gamma-glutamyl cycle. This pathway is central to maintaining intracellular glutathione levels and protecting cells from oxidative damage. The GGCT antibody provides researchers with a reliable reagent for studying redox balance, metabolic regulation, and stress response mechanisms.\u003cbr\u003e\u003cbr\u003eGamma-glutamylcyclotransferase localizes primarily to the cytosol, where it regulates the degradation of glutathione conjugates. By catalyzing gamma-glutamyl bond cleavage, the enzyme supports the recycling of amino acids used for glutathione synthesis. The GGCT antibody enables visualization and quantification of this enzyme in cells and tissues, supporting research into oxidative stress–related pathways and cellular detoxification processes. Through its activity, GGCT contributes to maintaining redox homeostasis and protecting against reactive oxygen species (ROS) accumulation.\u003cbr\u003e\u003cbr\u003eUpregulation of Gamma-glutamylcyclotransferase has been reported in various cancers, including breast, lung, and bladder tumors, where it is associated with enhanced proliferation and stress resistance. Elevated GGCT expression supports tumor growth by maintaining redox stability and promoting metabolic flexibility. The GGCT antibody is used to investigate these oncogenic functions, providing insight into how altered glutathione metabolism contributes to tumorigenesis. It also enables assessment of GGCT as a potential biomarker or therapeutic target in oxidative stress–driven cancers.\u003cbr\u003e\u003cbr\u003eBeyond cancer, Gamma-glutamylcyclotransferase plays roles in neuroprotection and metabolic adaptation. Its regulation affects glutathione turnover in the brain, liver, and kidneys, influencing detoxification capacity and antioxidant defense. The GGCT antibody allows researchers to track expression changes in response to nutritional status, xenobiotic exposure, and oxidative injury. Dysregulation of GGCT may contribute to neurodegenerative diseases and metabolic disorders characterized by redox imbalance.\u003cbr\u003e\u003cbr\u003eThe GGCT antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing strong cytoplasmic staining consistent with its enzymatic localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GGCT - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GGCT - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GGCT - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GGCT - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GGCT+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287251309,"sku":"FY12401","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5eff792f-607a-46a6-8258-8742593d9d76.jpg?v=1782237011","url":"https:\/\/www.ebiohippo.com\/products\/ggct-antibody-gamma-glutamylcyclotransferase-bha17135304","provider":"BioHippo","version":"1.0","type":"link"}