{"product_id":"ggh-antibody-gamma-glutamyl-hydrolase-bha17135567","title":"GGH Antibody \/ Gamma glutamyl hydrolase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGGH Antibody \/ Gamma glutamyl hydrolase is a anti-GGH Rabbit antibody Recombinant Rabbit Monoclonal clone 31G17 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GGH\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31G17, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGGH antibody detects gamma glutamyl hydrolase, an enzyme encoded by the GGH gene. GGH hydrolyzes polyglutamylated folates and antifolate drugs, converting them into monoglutamyl forms that can be transported across membranes. This activity regulates intracellular folate homeostasis and influences the efficacy of antifolate chemotherapeutics such as methotrexate. By controlling folate metabolism, GGH contributes to nucleotide synthesis, DNA replication, and cell proliferation.\u003cbr\u003e\u003cbr\u003eGGH antibody is widely applied in oncology, pharmacology, and metabolism research. Elevated GGH expression reduces the retention and potency of antifolate drugs, contributing to chemoresistance in leukemia and solid tumors. Conversely, altered GGH activity affects folate availability and one carbon metabolism, impacting DNA synthesis and repair. By detecting GGH, researchers can study how folate metabolism intersects with cancer biology, drug resistance, and metabolic disease.\u003cbr\u003e\u003cbr\u003e Western blot assays detect GGH protein bands in liver and tumor extracts. Immunohistochemistry maps expression in proliferative tissues and tumors, while immunofluorescence highlights cytoplasmic localization in cells actively synthesizing DNA. These approaches provide insight into how GGH distribution correlates with metabolic activity and drug response.\u003cbr\u003e\u003cbr\u003eGGH also plays roles in normal physiology, regulating folate supply for rapidly dividing cells in bone marrow, intestinal epithelium, and developing tissues. Dysregulation of GGH has been associated with megaloblastic anemia, cancer progression, and sensitivity to antifolate therapy. By applying GGH antibody, scientists can explore the balance between folate metabolism and proliferation in both health and disease.\u003cbr\u003e\u003cbr\u003eGGH antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GGH - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GGH - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GGH - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GGH - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GGH+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047296131437,"sku":"FY12664","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_45c4a388-05c7-45e2-8655-17ce0814ea0e.jpg?v=1772019326","url":"https:\/\/www.ebiohippo.com\/products\/ggh-antibody-gamma-glutamyl-hydrolase-bha17135567","provider":"BioHippo","version":"1.0","type":"link"}