{"product_id":"gins2-antibody-gins-protein-subunit-2-psf2-bha17136109","title":"GINS2 Antibody \/ GINS protein subunit 2 \/ PSF2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGINS2 Antibody \/ GINS protein subunit 2 \/ PSF2 is a anti-GINS2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GINS2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGINS2 antibody detects DNA replication complex GINS protein subunit 2, also known as PSF2, a component of the GINS complex that is essential for the initiation and elongation of DNA replication. The UniProt recommended name is DNA replication complex GINS protein subunit 2 (GINS2). This protein functions as part of a heterotetrameric complex composed of GINS1 (Sld5), GINS2 (Psf2), GINS3 (Psf3), and GINS4 (Sld15), which together interact with the MCM helicase and CDC45 to form the active CMG (Cdc45-MCM-GINS) helicase complex.\u003cbr\u003e\u003cbr\u003eFunctionally, GINS2 antibody identifies a 204-amino-acid nuclear protein required for the progression of DNA replication forks. GINS2 promotes helicase activation, stabilizes replication machinery, and facilitates the unwinding of DNA strands during S-phase. By interacting with key replication factors including MCM2-7, CDC45, and DNA polymerase alpha, GINS2 ensures accurate and efficient genome duplication. It also contributes to replication checkpoint control, preventing DNA damage accumulation and maintaining genomic stability.\u003cbr\u003e\u003cbr\u003eThe GINS2 gene is located on chromosome 16q24.1 and is expressed in proliferating tissues such as bone marrow, thymus, and tumors. Expression is cell cycle-regulated, peaking during S-phase when DNA replication is most active. GINS2 is evolutionarily conserved, highlighting its fundamental role in DNA synthesis and cell division.\u003cbr\u003e\u003cbr\u003ePathologically, GINS2 overexpression has been linked to tumorigenesis and poor prognosis in cancers including breast, lung, and gastric carcinoma. Excessive GINS2 activity enhances cell proliferation, while its depletion leads to replication stress, checkpoint activation, and apoptosis. Research using GINS2 antibody supports studies in DNA replication, cell cycle regulation, and cancer biology.\u003cbr\u003e\u003cbr\u003eGINS2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect replication complex components. \u003cbr\u003e\u003cbr\u003eStructurally, DNA replication complex GINS protein subunit 2 adopts a beta-strand-rich conformation that contributes to the ring-shaped architecture of the GINS complex. This structural organization provides stability and interaction surfaces for other replication proteins. This antibody facilitates detailed investigation of GINS2's mechanistic role in replication fork progression and cancer-related replication stress.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GINS2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GINS2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GINS2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GINS2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GINS2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047318479213,"sku":"FY13207","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5fe29304-22ce-4ffd-95c3-b8bb1c57b8d4.jpg?v=1782237084","url":"https:\/\/www.ebiohippo.com\/products\/gins2-antibody-gins-protein-subunit-2-psf2-bha17136109","provider":"BioHippo","version":"1.0","type":"link"}