{"product_id":"gins3-antibody-gins-protein-subunit-3-bha17135938","title":"GINS3 Antibody \/ GINS protein subunit 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGINS3 Antibody \/ GINS protein subunit 3 is a anti-GINS3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GINS3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGINS3 antibody detects DNA replication complex GINS protein subunit 3, a component of the GINS complex essential for the initiation and elongation phases of eukaryotic DNA replication. The UniProt recommended name is DNA replication complex GINS protein subunit 3 (GINS3). This protein is a critical element of the CMG helicase complex, which unwinds DNA strands during replication fork progression.\u003cbr\u003e\u003cbr\u003eFunctionally, GINS3 antibody identifies a 223-amino-acid protein that forms part of the heterotetrameric GINS complex along with GINS1 (Sld5), GINS2 (Psf2), and GINS4 (Psf3). The GINS complex interacts with Cdc45 and the MCM2-7 helicase to form the CMG (Cdc45-MCM-GINS) complex, which drives replication fork unwinding and DNA synthesis. GINS3 stabilizes this complex, ensuring efficient replication initiation and fork progression during S phase.\u003cbr\u003e\u003cbr\u003eThe GINS3 gene is located on chromosome 16q24.1 and encodes a conserved protein expressed in proliferating cells and tissues undergoing rapid growth. GINS3 functions as a scaffold subunit, mediating interactions between GINS partners and replication factors such as DNA polymerase epsilon. Its depletion leads to stalled replication forks, DNA damage accumulation, and activation of checkpoint signaling, highlighting its essential role in genome duplication.\u003cbr\u003e\u003cbr\u003eIn cell cycle regulation, GINS3 contributes to origin firing and replication timing control. It is recruited to replication origins during early S phase and remains associated with active replication forks. Dysregulation of GINS3 or other CMG components disrupts DNA replication dynamics and can result in replication stress, chromosomal instability, and tumorigenesis. Overexpression of GINS subunits has been observed in cancers characterized by high proliferation rates, including breast, ovarian, and colorectal cancers.\u003cbr\u003e\u003cbr\u003eGINS3 antibody is widely used in cell cycle, DNA replication, and cancer research. It is suitable for immunoblotting, immunofluorescence, and chromatin fractionation assays to examine GINS3 expression and complex formation. This antibody supports studies of replication fork mechanics, checkpoint signaling, and DNA damage responses. In oncology, it helps assess replication stress pathways and therapeutic sensitivity to DNA synthesis inhibitors.\u003cbr\u003e\u003cbr\u003eStructurally, GINS3 adopts a beta-sheet-rich fold that interfaces with GINS1 and GINS2 to stabilize the quaternary complex. It acts as a bridge linking DNA helicase and polymerase activities at replication forks.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GINS3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GINS3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GINS3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GINS3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GINS3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310483821,"sku":"FY13036","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3a5ccdfc-adfd-4d4f-8ae6-dcccc701f31c.jpg?v=1782237076","url":"https:\/\/www.ebiohippo.com\/products\/gins3-antibody-gins-protein-subunit-3-bha17135938","provider":"BioHippo","version":"1.0","type":"link"}