{"product_id":"gja4-antibody-gap-junction-alpha-4-bha17135509","title":"GJA4 Antibody \/ Gap junction alpha 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGJA4 Antibody \/ Gap junction alpha 4 is a anti-GJA4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GJA4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGJA4 antibody detects Gap junction alpha-4 protein, also known as Connexin 37, a transmembrane protein forming intercellular channels that mediate direct communication between adjacent cells. GJA4 enables diffusion of ions, metabolites, and signaling molecules, maintaining vascular tone and tissue homeostasis. The GJA4 antibody is widely used in cardiovascular, reproductive, and cellular communication studies to explore gap junction biology and endothelial signaling.\u003cbr\u003e\u003cbr\u003eGJA4 is encoded by the GJA4 gene on human chromosome 1p35.1. The protein is approximately 333 amino acids long and contains four transmembrane helices, two extracellular loops, and cytoplasmic N- and C-terminal domains. GJA4 assembles into hexameric connexons that dock with connexons of neighboring cells, forming gap junction channels that permit electrical and chemical coupling.\u003cbr\u003e\u003cbr\u003eThe GJA4 antibody detects a 37 kilodalton band by western blot and shows punctate membrane staining consistent with gap junction plaques under immunofluorescence. GJA4 is abundantly expressed in vascular endothelial and smooth muscle cells, where it mediates synchronized contraction and vascular tone regulation. In ovarian follicles and the placenta, GJA4 facilitates cell communication critical for follicular development and embryonic exchange.\u003cbr\u003e\u003cbr\u003eMutations in GJA4 disrupt gap junction communication, contributing to vascular dysfunction and reproductive disorders. Altered GJA4 expression has been observed in atherosclerosis, hypertension, and tumor angiogenesis. Its expression pattern varies with hormonal and mechanical stimuli, reflecting its responsiveness to physiological cues controlling vascular integrity.\u003cbr\u003e\u003cbr\u003eGJA4 channels are regulated by phosphorylation, pH, and redox state, allowing dynamic modulation of intercellular connectivity. Because gap junctions coordinate tissue-level responses, GJA4 represents a vital component of multicellular signaling networks.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GJA4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GJA4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GJA4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GJA4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GJA4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047293968749,"sku":"FY12606","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ac7ca584-9d10-406b-a7e8-c3351eb78a37.jpg?v=1782237018","url":"https:\/\/www.ebiohippo.com\/products\/gja4-antibody-gap-junction-alpha-4-bha17135509","provider":"BioHippo","version":"1.0","type":"link"}