{"product_id":"gk5-antibody-glycerol-kinase-5-bha17135327","title":"GK5 Antibody \/ Glycerol kinase 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGK5 Antibody \/ Glycerol kinase 5 is a anti-GK5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GK5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe GK5 antibody targets Glycerol kinase 5, a mitochondrial and peroxisomal enzyme encoded by the GK5 gene. Glycerol kinase 5 phosphorylates glycerol to glycerol-3-phosphate, a key intermediate in lipid metabolism and energy homeostasis. As a member of the glycerol kinase family, this enzyme contributes to triglyceride synthesis, fatty-acid metabolism, and membrane lipid turnover. The GK5 antibody provides a reliable reagent for studying glycerol utilization, lipid signaling, and mitochondrial metabolic integration.\u003cbr\u003e\u003cbr\u003eGlycerol kinase 5 is distinct from the canonical cytosolic glycerol kinase (GK1) in its subcellular localization and tissue specificity. It is highly expressed in testis and skin, where it may regulate lipid composition during spermatogenesis and barrier formation. The GK5 antibody allows detection of this enzyme in mitochondria-rich tissues, helping define its contribution to energy production and lipid biosynthesis. By phosphorylating glycerol, GK5 supports the glycerol phosphate shuttle and links carbohydrate metabolism with lipid anabolism.\u003cbr\u003e\u003cbr\u003eStructurally, Glycerol kinase 5 shares conserved ATP-binding and catalytic residues with other glycerol kinases but contains mitochondrial targeting sequences. It associates with mitochondrial membranes, where it modulates local glycerol metabolism. The GK5 antibody supports subcellular localization studies that examine mitochondrial lipid synthesis and oxidative phosphorylation coupling. In skin and reproductive tissues, GK5 may play roles in lipid barrier maintenance and sperm energy metabolism.\u003cbr\u003e\u003cbr\u003eAltered expression of Glycerol kinase 5 has been observed in metabolic disorders and certain cancers. Its involvement in lipid signaling suggests that GK5 activity could affect membrane dynamics and cellular energy balance. The GK5 antibody facilitates quantification of expression changes under metabolic stress, nutrient deprivation, or differentiation. Functional studies indicate that GK5 may participate in adaptive metabolic responses that sustain energy production under shifting substrate conditions.\u003cbr\u003e\u003cbr\u003eThe GK5 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing punctate mitochondrial staining consistent with its predicted localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GK5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GK5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GK5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GK5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GK5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287153005,"sku":"FY12424","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f9cbc7da-172c-47e7-8894-a5e245ac6a93.jpg?v=1782237003","url":"https:\/\/www.ebiohippo.com\/products\/gk5-antibody-glycerol-kinase-5-bha17135327","provider":"BioHippo","version":"1.0","type":"link"}