{"product_id":"gkap1-antibody-g-kinase-anchoring-protein-1-bha17135483","title":"GKAP1 Antibody \/ G kinase-anchoring protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGKAP1 Antibody \/ G kinase-anchoring protein 1 is a anti-GKAP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GKAP1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGKAP1 antibody detects G kinase-anchoring protein 1, a scaffold protein that coordinates the localization and activity of cGMP-dependent protein kinase (PKG) signaling complexes. GKAP1 serves as a key adaptor linking kinases, phosphatases, and cytoskeletal elements, thereby influencing cellular responses to cyclic nucleotide signaling. The GKAP1 antibody is widely used in cardiovascular, renal, and neurobiology research to study cGMP signaling pathways and subcellular kinase organization.\u003cbr\u003e\u003cbr\u003eGKAP1 is encoded by the GKAP1 gene located on human chromosome 9q31.1. The protein is approximately 96 kilodaltons and characterized by multiple ankyrin repeats and leucine zipper motifs that mediate protein-protein interactions. It functions as an anchoring protein for PKG, positioning the kinase in proximity to its substrates at specific subcellular compartments, including the cytoskeleton and plasma membrane. This compartmentalization ensures spatial precision in cGMP-mediated signaling events.\u003cbr\u003e\u003cbr\u003eThe GKAP1 antibody detects a 96 kilodalton band by western blot and shows cytoplasmic and perinuclear localization under immunofluorescence. GKAP1 binds directly to PKG I and II isoforms, facilitating phosphorylation of target proteins involved in smooth muscle relaxation, ion channel regulation, and metabolic control. Through its anchoring role, GKAP1 contributes to compartmentalized cGMP signaling, distinguishing localized kinase activity from global cyclic nucleotide effects.\u003cbr\u003e\u003cbr\u003eBeyond its role in vascular signaling, GKAP1 participates in neuronal signaling and synaptic plasticity. It interacts with cytoskeletal proteins and actin-regulatory molecules, influencing axon growth and dendritic morphology. GKAP1 may also modulate insulin signaling and energy metabolism through PKG-mediated pathways in adipocytes and hepatocytes.\u003cbr\u003e\u003cbr\u003eDysregulation of GKAP1 expression alters cGMP signaling fidelity and is associated with cardiovascular disorders, hypertension, and metabolic diseases. Overexpression enhances PKG activity and smooth muscle relaxation, whereas loss of GKAP1 results in impaired vasodilation and reduced cellular responsiveness to nitric oxide. Because of its central role in cGMP signaling scaffolding, GKAP1 represents a valuable target for understanding kinase compartmentalization and cyclic nucleotide signaling networks.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GKAP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GKAP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GKAP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GKAP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GKAP1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047293215085,"sku":"FY12580","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3c929e1c-dd43-40be-987e-4b4059387d3a.jpg?v=1782237019","url":"https:\/\/www.ebiohippo.com\/products\/gkap1-antibody-g-kinase-anchoring-protein-1-bha17135483","provider":"BioHippo","version":"1.0","type":"link"}