{"product_id":"gkn1-antibody-gastrokine-1-bha17135614","title":"GKN1 Antibody \/ Gastrokine 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGKN1 Antibody \/ Gastrokine 1 is a anti-GKN1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse. Reported localization: Cytoplasm, Golgi, secreted.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GKN1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGKN1 antibody detects Gastrokine-1 (also known as Antral mucosal protein or AMP-18), a secreted epithelial protein that promotes gastric mucosal integrity and repair. Encoded by the GKN1 gene on chromosome 2p13.3, this small peptide is abundantly expressed in the stomach epithelium, where it supports epithelial cell survival, migration, and restitution following injury. Gastrokine-1 contains a BRICHOS domain that mediates protein-protein interactions involved in epithelial protection and regeneration. It is secreted into the gastric lumen and acts both locally and paracrinally to maintain mucosal homeostasis.\u003cbr\u003e\u003cbr\u003eGKN1 expression is restricted to normal gastric mucosa and is markedly downregulated or lost in gastric cancer, making it a useful biomarker for gastric differentiation and neoplastic transformation. Functional studies demonstrate that GKN1 suppresses gastric tumor cell proliferation, modulates NF-kB signaling, and inhibits epithelial-mesenchymal transition. Loss of GKN1 leads to increased susceptibility to inflammation-induced damage and carcinogenesis. By promoting mucosal healing and preserving epithelial barrier integrity, GKN1 functions as a tumor suppressor and protective factor in the gastrointestinal tract.\u003cbr\u003e\u003cbr\u003eThe GKN1 antibody is widely used in gastrointestinal biology, cancer, and inflammation research to detect Gastrokine-1 in tissue sections or cell lysates. In immunohistochemistry, GKN1 localizes predominantly to the cytoplasm and secretory granules of surface mucous cells. Western blotting identifies a 19 kilodalton band corresponding to the mature secreted form. The antibody assists in distinguishing normal gastric tissue from malignant lesions, as GKN1 expression is largely absent in gastric adenocarcinoma but retained in non-neoplastic mucosa. This differential expression pattern has diagnostic and prognostic implications.\u003cbr\u003e\u003cbr\u003eBeyond the stomach, low levels of GKN1 are occasionally detected in duodenal and pancreatic tissues, suggesting secondary protective roles in the upper gastrointestinal tract. The protein also exhibits antibacterial activity and modulates host responses to Helicobacter pylori infection. Because of these diverse functions, the GKN1 antibody supports research into epithelial regeneration, cancer suppression, and gastric disease pathogenesis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GKN1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GKN1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GKN1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GKN1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GKN1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296950637,"sku":"FY12711","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d1f0f9c6-3346-47c5-b48b-9ce8cc9be3d2.jpg?v=1782237040","url":"https:\/\/www.ebiohippo.com\/products\/gkn1-antibody-gastrokine-1-bha17135614","provider":"BioHippo","version":"1.0","type":"link"}