{"product_id":"gli-reporter-lentivirus-hedgehog-pathway-bhv19400011","title":"GLI Reporter Lentivirus (Hedgehog Pathway)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Hedgehog signaling pathway is a key regulator of embryonic development, tissue patterning, and adult stem cell maintenance. It is initiated by Hedgehog ligands, including Sonic (Shh), Indian (Ihh), and Desert (Dhh) hedgehog, which bind the receptor Patched and relieve its inhibition of Smoothened. Activated signaling converges on the GLI family of zinc-finger transcription factors, which bind consensus GLI response elements to control target gene expression. The family comprises the activator GLI1 and the dual activator-repressor factors GLI2 and GLI3. Aberrant Hedgehog\/GLI signaling drives several cancers, including basal cell carcinoma and medulloblastoma, making this pathway an important focus of developmental and oncology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe GLI Reporter Lentivirus places a reporter gene under the control of tandem consensus GLI response elements, providing a sensitive readout of Hedgehog pathway activity in mammalian cells. Reporter options include fluorescent (GFP, RFP) and luminescent (firefly and Renilla luciferase) formats, with blasticidin or puromycin selection for stable reporter cell line establishment. Signal can be measured by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring GLI transcriptional activity in response to Hedgehog ligands, studying developmental signaling, and screening Hedgehog pathway agonists and inhibitors.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the GLI\/Hedgehog pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251618898285,"sku":"LTV-0012-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310895227245,"sku":"LTV-0012-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310895260013,"sku":"LTV-0012-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310895292781,"sku":"LTV-0012-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310895325549,"sku":"LTV-0012-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310895358317,"sku":"LTV-0012-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310895391085,"sku":"LTV-0012-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_7f886f25-c1bb-4850-806e-555d9d70093d.jpg?v=1776934270","url":"https:\/\/www.ebiohippo.com\/products\/gli-reporter-lentivirus-hedgehog-pathway-bhv19400011","provider":"BioHippo","version":"1.0","type":"link"}