{"product_id":"gli1-antibody-glioma-associated-oncogene-homolog-1-bha17135095","title":"GLI1 Antibody \/ Glioma-associated oncogene homolog 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGLI1 Antibody \/ Glioma-associated oncogene homolog 1 is an antibody targeting \u003cstrong\u003eGLI1\u003c\/strong\u003e. Host: \u003cstrong\u003eRabbit\u003c\/strong\u003e. Polyclonal. Format: Lyophilized. Applications: WB, ELISA.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GLI1.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e E.coli-derived human GLI1 recombinant protein (Position: Y800-Q1057) was used as the immunogen for the GLI1 antibody..\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human, Mouse, Rat.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate:\u003c\/strong\u003e Unconjugated (no label listed).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background (from provided description)\u003c\/h2\u003e\u003cp\u003eGLI1 antibody detects Glioma-associated oncogene homolog 1, encoded by the GLI1 gene on chromosome 12q13.3. GLI1 antibody is used in research on developmental signaling, transcriptional regulation, and cancer biology. GLI1 is a zinc finger transcription factor and a key effector of the Hedgehog signaling pathway, which controls cell fate, proliferation, and differentiation during embryogenesis.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWB:\u003c\/strong\u003e relative abundance and band pattern assessment using appropriate controls.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAccount for isoforms, paralogs, and post-translational modifications when interpreting signal.\u003c\/li\u003e\n\u003cli\u003eSupport specificity with biological controls (e.g., KO\/KD where available) and technical controls (e.g., secondary-only\/isotype).\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt Knowledgebase (UniProt) – https:\/\/www.uniprot.org\/ - NCBI Gene (NIH\/NLM) – https:\/\/www.ncbi.nlm.nih.gov\/gene\/ - ZFIN – https:\/\/zfin.org\/ - Ensembl – https:\/\/www.ensembl.org\/ - Nature Methods (2015): A proposal for validation of antibodies – https:\/\/www.nature.com\/articles\/nmeth.3230 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53046718595437,"sku":"FY12192","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_40678213-6945-4c96-8e85-919a48ad59af.jpg?v=1782236976","url":"https:\/\/www.ebiohippo.com\/products\/gli1-antibody-glioma-associated-oncogene-homolog-1-bha17135095","provider":"BioHippo","version":"1.0","type":"link"}