{"product_id":"global-histone-h3k4-methylation-assay-kit-bht19600004","title":"Global Histone H3K4 Methylation Assay Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;border:1px solid #ccd9f0}.bhp-desc td{padding:8px 12px;border:1px solid #ccd9f0}.bhp-desc .pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc .warn{background:#fff6ee;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"pill\"\u003eAssay Kit\u003c\/span\u003e\u003cp\u003eH3K4 methylation refers to the addition of methyl groups to the lysine 4 residue of histone H3, a process that can occur in three states: mono-, di-, and trimethylation (H3K4me1, H3K4me2, and H3K4me3). This modification is associated with transcriptional activation and is typically found at the promoter and enhancer regions of actively transcribed genes. H3K4 methylation is a conserved chromatin modification across various organisms, from yeast to humans. H3K4 methylation plays a critical role in gene expression regulation, chromatin structure, and various biological processes, including development and differentiation. The Global Histone H3K4 Methylation Assay is designed to measure H3-K4 methylation in various mammalian cells, including human and mouse cells, as well as fresh and frozen tissues, cultured adherent cells, and suspension cells. This assay is simple, accurate, and fast. The measurement can be completed in 5 hours, with a recommended sample input of 5 ng to 2 µg per well.\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThe Global Histone H3K4 Methylation Assay Kit is a immunoassay for the quantitative measurement of methylated histone h3k4 in mammalian cells and tissues. Target histones are captured on a pre-coated microplate and detected with a modification-specific antibody, then quantified against a standard curve. The assay measures global histone h3k4 methylation with a recommended sample input of 5 ng – 2 µg per well and can be completed in 5 hours.\u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cp\u003eThe kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (modification-specific antibody and enzyme\/fluorophore conjugate), wash buffer, and developing\/stop solutions. Refer to the product datasheet for the full component list and volumes.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Target\u003c\/th\u003e\n\u003ctd\u003eGlobal histone H3K4 methylation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnalyte\u003c\/th\u003e\n\u003ctd\u003eMethylated Histone H3K4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eImmunosorbent assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Time\u003c\/th\u003e\n\u003ctd\u003e5 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecommended Sample Input\u003c\/th\u003e\n\u003ctd\u003e5 ng – 2 µg per well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eHuman, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Compatibility\u003c\/th\u003e\n\u003ctd\u003eMammalian cells (human, mouse); fresh \u0026amp; frozen tissues; adherent \u0026amp; suspension cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore the unopened kit at 2–8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"warn\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices and consult the SDS before handling.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"1 x 48-well plate","offer_id":53550126367085,"sku":"TBS32123-48","price":640.0,"currency_code":"USD","in_stock":true},{"title":"1 x 96-well plate","offer_id":53550126530925,"sku":"TBS32123-96","price":952.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TBS32123.png?v=1784060059","url":"https:\/\/www.ebiohippo.com\/products\/global-histone-h3k4-methylation-assay-kit-bht19600004","provider":"BioHippo","version":"1.0","type":"link"}