{"product_id":"glod4-antibody-glyoxalase-domain-containing-protein-4-bha17135221","title":"GLOD4 Antibody \/ Glyoxalase domain-containing protein 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGLOD4 Antibody \/ Glyoxalase domain-containing protein 4 is a anti-GLOD4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GLOD4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGLOD4 antibody detects Glyoxalase domain-containing protein 4, encoded by the GLOD4 gene on chromosome 17q25.3. GLOD4 antibody is widely applied in metabolism, detoxification, and mitochondrial biology research. Although GLOD4's precise enzymatic role remains incompletely defined, it contains homology to glyoxalase enzymes, which detoxify reactive dicarbonyls such as methylglyoxal, a byproduct of glycolysis.\u003cbr\u003e\u003cbr\u003eStructurally, GLOD4 is a ~36 kDa protein with a glyoxalase-like fold. It is predicted to bind metal ions that assist catalysis, though definitive enzymatic activity has yet to be confirmed. Localization studies suggest enrichment in mitochondria, highlighting a role in redox balance and metabolic regulation.\u003cbr\u003e\u003cbr\u003eFunctionally, GLOD4 may protect cells from glycation stress by metabolizing reactive aldehydes. Its expression is broad, with higher levels in metabolic tissues such as liver, kidney, and muscle. Researchers use GLOD4 antibody to study mitochondrial metabolism, detoxification pathways, and oxidative stress responses.\u003cbr\u003e\u003cbr\u003eClinically, altered GLOD4 expression has been observed in cancer and metabolic disease. By influencing redox balance, GLOD4 may contribute to tumor survival under stress conditions. It is under investigation as a metabolic biomarker and therapeutic target. \u003cbr\u003e\u003cbr\u003eExperimentally, GLOD4 antibody is applied in western blotting to detect the ~36 kDa protein, in immunofluorescence microscopy to study mitochondrial localization, and in immunohistochemistry to assess expression across tissues. Functional studies combining GLOD4 antibody with metabolic assays help clarify its enzymatic role in glyoxalase pathways.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GLOD4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GLOD4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GLOD4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GLOD4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GLOD4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047284760941,"sku":"FY12318","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_60b72738-7789-4126-bd24-1577975c1e0a.jpg?v=1782236991","url":"https:\/\/www.ebiohippo.com\/products\/glod4-antibody-glyoxalase-domain-containing-protein-4-bha17135221","provider":"BioHippo","version":"1.0","type":"link"}