{"product_id":"glod5-antibody-glyoxalase-domain-containing-protein-5-bha17135980","title":"GLOD5 Antibody \/ Glyoxalase domain-containing protein 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGLOD5 Antibody \/ Glyoxalase domain-containing protein 5 is a anti-GLOD5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Mitochondria.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GLOD5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGLOD5 antibody detects Glyoxalase domain-containing protein 5, an oxidoreductase-like protein potentially involved in detoxification of reactive carbonyl compounds. The UniProt recommended name is Glyoxalase domain-containing protein 5 (GLOD5). Although its enzymatic activity remains incompletely characterized, sequence analysis indicates homology to the glyoxalase and dioxygenase enzyme families responsible for cellular stress defense.\u003cbr\u003e\u003cbr\u003eFunctionally, GLOD5 antibody recognizes a cytoplasmic protein of approximately 160 amino acids that contains a metalloenzyme fold typical of glyoxalase domains. It is thought to play a role in the metabolism of reactive aldehydes and other cytotoxic intermediates generated during oxidative stress. GLOD5 expression may also contribute to maintaining redox balance and protecting cells from metabolic byproducts.\u003cbr\u003e\u003cbr\u003eThe GLOD5 gene is located on chromosome 9p13.2 and exhibits widespread expression in human tissues, including brain, kidney, and liver. Its evolutionary conservation among mammals suggests a physiological role in small-molecule metabolism or detoxification pathways. GLOD5 expression has been reported to increase under oxidative stress conditions, further linking it to cellular protection mechanisms.\u003cbr\u003e\u003cbr\u003ePathologically, altered GLOD5 expression may influence cell viability and stress adaptation. Dysregulation of glyoxalase-related enzymes is associated with diabetes, neurodegeneration, and cancer, though the specific contribution of GLOD5 remains under investigation. Using GLOD5 antibody enables detection of this lesser-known member of the glyoxalase family and aids exploration of its potential metabolic roles.\u003cbr\u003e\u003cbr\u003eGLOD5 antibody is suitable for western blotting, immunocytochemistry, and immunohistochemistry to detect GLOD5 in cell and tissue extracts. \u003cbr\u003e\u003cbr\u003eStructurally, GLOD5 contains a glyoxalase-like catalytic fold capable of coordinating divalent metal ions, although its active-site residues are not fully conserved. This antibody assists researchers in clarifying GLOD5's molecular function and contribution to oxidative metabolism.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GLOD5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GLOD5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GLOD5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GLOD5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GLOD5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047311761773,"sku":"FY13078","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_14a3fd13-4c09-4e02-880a-41235cdac48a.jpg?v=1782237082","url":"https:\/\/www.ebiohippo.com\/products\/glod5-antibody-glyoxalase-domain-containing-protein-5-bha17135980","provider":"BioHippo","version":"1.0","type":"link"}