{"product_id":"glrx3-antibody-glutaredoxin-3-picot-bha17135625","title":"GLRX3 Antibody \/ Glutaredoxin 3 \/ PICOT","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGLRX3 Antibody \/ Glutaredoxin 3 \/ PICOT is a anti-GLRX3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GLRX3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGLRX3 antibody detects Glutaredoxin-3 (also known as PICOT or Thioredoxin-like protein 2), a multifunctional redox enzyme that regulates cellular redox homeostasis, iron-sulfur cluster assembly, and signal transduction. Encoded by the GLRX3 gene on chromosome 10q26.3, this protein belongs to the glutaredoxin family, characterized by their thiol-disulfide oxidoreductase activity. GLRX3 contains two monothiol glutaredoxin domains that utilize glutathione to catalyze reversible thiol exchange reactions, thereby maintaining protein thiol status and redox balance. Beyond redox control, GLRX3 acts as a scaffold protein for Fe-S cluster delivery and as a modulator of intracellular signaling cascades.\u003cbr\u003e\u003cbr\u003eGLRX3 is ubiquitously expressed but particularly abundant in heart, skeletal muscle, and liver, where it contributes to mitochondrial function and oxidative stress resistance. The protein forms complexes with BolA-like proteins to facilitate Fe-S cluster biogenesis, which is essential for electron transport chain integrity. In cardiomyocytes, GLRX3�originally identified as PICOT (PKC-interacting cousin of thioredoxin)�binds to protein kinase C theta and inhibits its activity, modulating hypertrophic signaling and contractility. This redox-regulatory interaction links GLRX3 to cardiac protection against oxidative damage and maladaptive stress responses.\u003cbr\u003e\u003cbr\u003eThe GLRX3 antibody is widely used in redox biology, metabolism, and cardiovascular research to examine oxidative stress regulation, Fe-S cluster metabolism, and cell signaling. Western blot analysis identifies a 38 kilodalton band corresponding to GLRX3, while immunofluorescence shows cytoplasmic and mitochondrial localization. Studies using the antibody have revealed that GLRX3 depletion leads to increased ROS levels, impaired mitochondrial respiration, and reduced iron homeostasis. Because these effects contribute to cellular senescence and disease progression, GLRX3 is of growing interest as a therapeutic target in oxidative and metabolic disorders.\u003cbr\u003e\u003cbr\u003eBeyond its roles in redox and energy metabolism, GLRX3 contributes to developmental processes and immune function. It modulates NF-?B and MAPK pathways, influencing inflammatory gene expression. Overexpression has been reported in cancers such as hepatocellular carcinoma and breast carcinoma, where it promotes proliferation and resistance to oxidative stress. The GLRX3 antibody therefore provides a valuable reagent for investigating molecular mechanisms of redox regulation, signal transduction, and disease adaptation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GLRX3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GLRX3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GLRX3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GLRX3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GLRX3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047298294125,"sku":"FY12722","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_bea3f7e8-3be0-4a3b-9a47-344125421e1b.jpg?v=1782237032","url":"https:\/\/www.ebiohippo.com\/products\/glrx3-antibody-glutaredoxin-3-picot-bha17135625","provider":"BioHippo","version":"1.0","type":"link"}