{"product_id":"glud1-antibody-glutamate-dehydrogenase-1-bha17135890","title":"GLUD1 Antibody \/ Glutamate dehydrogenase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGLUD1 Antibody \/ Glutamate dehydrogenase 1 is a anti-GLUD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria, ER).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GLUD1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IP, IF, IHC, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGLUD1 antibody detects Glutamate dehydrogenase 1, a mitochondrial enzyme that catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. The UniProt recommended name is Glutamate dehydrogenase 1, mitochondrial (GLUD1). This enzyme connects amino acid metabolism to the tricarboxylic acid (TCA) cycle, serving as a key regulator of nitrogen balance and cellular energy production.\u003cbr\u003e\u003cbr\u003eFunctionally, GLUD1 antibody identifies a 558-amino-acid enzyme located in the mitochondrial matrix. GLUD1 catalyzes the interconversion of glutamate and alpha-ketoglutarate with the reduction or oxidation of NAD(P)+ cofactors. This reaction controls the flow of carbon and nitrogen through metabolic networks, linking amino acid catabolism, the TCA cycle, and oxidative phosphorylation. In the brain, GLUD1 contributes to the glutamate-glutamine cycle, influencing neurotransmitter turnover and synaptic function.\u003cbr\u003e\u003cbr\u003eThe GLUD1 gene is located on chromosome 10q23.3 and encodes a hexameric enzyme regulated allosterically by ADP, GTP, leucine, and other metabolites. GLUD1 activity increases during energy demand, providing substrates for ATP synthesis, while its inhibition by GTP prevents excessive ammonia production. Mutations in GLUD1 cause hyperinsulinism-hyperammonemia (HI\/HA) syndrome, characterized by dysregulated insulin secretion due to elevated oxidative deamination in pancreatic beta cells.\u003cbr\u003e\u003cbr\u003eIn hepatic tissue, GLUD1 participates in ammonia detoxification and urea synthesis by controlling the balance between glutamate oxidation and synthesis. In pancreatic islets, its activity modulates insulin release in response to amino acids. In neurons, GLUD1 provides energy intermediates and regulates excitatory neurotransmission. Dysregulation of GLUD1 is implicated in neurodegenerative diseases, metabolic syndromes, and hyperinsulinemic disorders.\u003cbr\u003e\u003cbr\u003eGLUD1 antibody is widely used in research involving metabolism, neurobiology, and endocrinology. It is suitable for immunoblotting, immunofluorescence, and enzyme localization studies to assess mitochondrial distribution and activity. This antibody enables detection of GLUD1 in tissues such as liver, brain, and pancreas, where it plays distinct metabolic roles. In cancer research, GLUD1 expression correlates with metabolic reprogramming, supporting tumor cell growth through glutamine oxidation.\u003cbr\u003e\u003cbr\u003eStructurally, GLUD1 forms a hexameric complex with alternating catalytic and regulatory domains. The enzyme's activity is modulated by phosphorylation, redox state, and energy charge.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GLUD1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GLUD1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GLUD1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GLUD1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GLUD1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047308419437,"sku":"FY12988","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0bc10713-e25d-4d49-a456-4da0a2064923.jpg?v=1772019417","url":"https:\/\/www.ebiohippo.com\/products\/glud1-antibody-glutamate-dehydrogenase-1-bha17135890","provider":"BioHippo","version":"1.0","type":"link"}