{"product_id":"gnao1-r243h-stably-expressing-immortalized-human-mammary-epithelial-cell-line-bhc10901174","title":"GNAO1 R243H Stably Expressing Immortalized Human Mammary Epithelial Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eGNAO1 R243H Stably Expressing Immortalized Human Mammary Epithelial Cell line expresses GNAO1, the protein of which represent the alpha subunit of the G0 heterotrimeric G-protein signal-transducing complex and is partially responsible for smooth muscle contraction and depressing neuronal activity. This genes role is to inhibit adenylate cyclase , open K -channels (via β\/γ subunits), and close Ca2 -channels.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e GNAO1 R243H Stably Expressing Immortalized Human Mammary Epithelial Cell Line is supplied as a tumor cell line derived from Human mammary.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 50 µM fresh ascorbic acid + 2 nM estradiol + 1 µg\/ml insulin + 2.8 µM hydrocortisone + 0.1 mM ethanolamine + 0.1 mM L-glutamine (G275), 15 nM sodium selenite + 1 ng\/ml cholera toxin + 12.5 ng\/ml hEGF (Z100139) + 10% fetal bovine serum (TM999) or 35 µg\/ml bovine pituitary extract (Hammond) + 1% Penicillin\/Streptomycin Solution (G255). Adjust media to pH 7.4 before use, 37.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Gfp reporter expression.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis particular cell line expresses the Arginine243 → Histidine (R243H) mutation of GNAO1, which has been identified in breast carcinomas and is known to promote oncogenesis when introduced into cells. The oncogenic R234H mutation renders Gαo constitutively active by accelerating the rate of nucleotide exchange, which enhances signaling pathways that are associated with neoplasmic transformation. This cell line was made using retrovirus transduction. It carries flag tag and expresses GFP. Donor\/background information provided for this product: Female.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 50 µM fresh ascorbic acid + 2 nM estradiol + 1 µg\/ml insulin + 2.8 µM hydrocortisone + 0.1 mM ethanolamine + 0.1 mM L-glutamine (G275), 15 nM sodium selenite + 1 ng\/ml cholera toxin + 12.5 ng\/ml hEGF (Z100139) + 10% fetal bovine serum (TM999) or 35 µg\/ml bovine pituitary extract (Hammond) + 1% Penicillin\/Streptomycin Solution (G255). Adjust media to pH 7.4 before use, 37.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 10,000 - 20,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180519186797,"sku":"T6202","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/WQNEt3pULTXfqdSXhmeGSQI6DcJjUjZg6ZFMPiMU.png?v=1774957803","url":"https:\/\/www.ebiohippo.com\/products\/gnao1-r243h-stably-expressing-immortalized-human-mammary-epithelial-cell-line-bhc10901174","provider":"BioHippo","version":"1.0","type":"link"}