{"product_id":"gnaq-antibody-guanine-nucleotide-binding-protein-gq-subunit-alpha-bha17135789","title":"GNAQ Antibody \/ Guanine nucleotide binding protein Gq subunit alpha","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGNAQ Antibody \/ Guanine nucleotide binding protein Gq subunit alpha is a anti-GNAQ Rabbit antibody Recombinant Rabbit Monoclonal clone 30G74 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus, Cytoplasm (Golgi), Cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GNAQ\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30G74, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGNAQ antibody detects Guanine nucleotide binding protein Gq subunit alpha, encoded by the GNAQ gene. This protein is a member of the heterotrimeric G protein alpha subunit family and couples receptors to downstream signaling cascades. Upon activation by G protein coupled receptors, GNAQ activates phospholipase C beta, leading to the production of inositol triphosphate and diacylglycerol. GNAQ antibody enables detailed study of this central signaling node and its contribution to cellular communication, growth, and differentiation.\u003cbr\u003e\u003cbr\u003eGuanine nucleotide binding protein Gq subunit alpha plays a pivotal role in calcium signaling, activating pathways that regulate smooth muscle contraction, secretion, and gene expression. Dysregulation of GNAQ activity has been linked to developmental disorders, vascular anomalies, and cancers. Mutations in GNAQ are frequent in uveal melanoma and Sturge Weber syndrome, where they drive aberrant MAPK signaling and cell proliferation. Research using GNAQ antibody provides a means to detect altered protein levels and examine how mutant forms disrupt signaling dynamics.\u003cbr\u003e\u003cbr\u003eStudies have also shown that GNAQ interacts with multiple effector pathways, including protein kinase C and Rho GTPases, extending its impact on cytoskeletal remodeling, migration, and survival. GNAQ antibody has been used to investigate signaling crosstalk in cardiovascular, neurological, and oncological contexts. Its function in coupling receptors to intracellular calcium release makes it indispensable for understanding receptor mediated physiology and pathology.\u003cbr\u003e\u003cbr\u003eGNAQ antibody is employed in western blotting, immunohistochemistry, and immunofluorescence. Western blotting measures expression in tissues such as brain, heart, and smooth muscle. Immunohistochemistry visualizes localization in vascular endothelium and tumor samples. Immunofluorescence reveals distribution at the plasma membrane, consistent with its role as a receptor coupled G protein. Functional experiments have used GNAQ antibody to track expression changes following pharmacological inhibition of GPCR pathways.\u003cbr\u003e\u003cbr\u003eBy providing validated GNAQ antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GNAQ - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GNAQ - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GNAQ - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GNAQ - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GNAQ+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047305306477,"sku":"FY12887","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5e1ec5be-ae05-42c4-a099-9af487694fad.jpg?v=1772019362","url":"https:\/\/www.ebiohippo.com\/products\/gnaq-antibody-guanine-nucleotide-binding-protein-gq-subunit-alpha-bha17135789","provider":"BioHippo","version":"1.0","type":"link"}