{"product_id":"gne-antibody-glucosamine-6-phosphate-n-acetyltransferase-bha17135253","title":"GNE Antibody \/ Glucosamine 6 phosphate N acetyltransferase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGNE Antibody \/ Glucosamine 6 phosphate N acetyltransferase is a anti-GNE Rabbit antibody Recombinant Rabbit Monoclonal clone 29G96 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GNE\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 29G96, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGNE antibody is designed to detect glucosamine 6 phosphate N acetyltransferase, a bifunctional enzyme involved in sialic acid biosynthesis. The GNE protein contains two enzymatic activities, UDP N acetylglucosamine 2 epimerase and N acetylmannosamine kinase. Together, these activities catalyze the first committed steps in the biosynthesis of N acetylneuraminic acid, the most common form of sialic acid in mammals. Sialic acids are critical components of glycoproteins and glycolipids, influencing cell surface interactions, receptor function, and immune regulation.\u003cbr\u003e\u003cbr\u003eGNE antibody is widely applied in metabolic, developmental, and neuromuscular research. Mutations in the GNE gene cause GNE myopathy, also known as hereditary inclusion body myopathy. This rare autosomal recessive disorder is characterized by progressive muscle weakness and degeneration. By using GNE antibody, researchers can investigate enzyme expression levels and localization to better understand the molecular basis of this disease.\u003cbr\u003e\u003cbr\u003eThe antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, GNE antibody specifically recognizes the protein across tissue samples, confirming its role in metabolic pathways. Immunohistochemistry highlights expression in muscle and epithelial tissues, while immunofluorescence demonstrates cytoplasmic localization within cultured cells. These approaches provide valuable insight into how GNE supports cellular glycosylation and metabolic balance.\u003cbr\u003e\u003cbr\u003eIn addition to its relevance in genetic disorders, GNE is important in cancer biology and immunology. Altered sialylation patterns are a hallmark of many cancers, where increased sialic acid residues on tumor cells promote immune evasion and metastasis. By monitoring GNE expression with specific antibodies, scientists can explore how dysregulation of sialic acid biosynthesis contributes to malignant transformation. Similarly, in immunology, GNE activity influences immune recognition, inflammation, and infection responses.\u003cbr\u003e\u003cbr\u003eSialic acids play essential roles in development and signaling. Disruption of GNE function affects not only muscle tissue but also systemic processes, highlighting the importance of reliable antibodies for detection. Using GNE antibody, researchers can explore how this key enzyme contributes to glycosylation pathways and how alterations affect health and disease.\u003cbr\u003e\u003cbr\u003eGNE antibody offered by\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GNE - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GNE - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GNE - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GNE - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GNE+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047286006125,"sku":"FY12350","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_66173bb5-bebc-48b1-8f4e-644c156c087c.jpg?v=1772019280","url":"https:\/\/www.ebiohippo.com\/products\/gne-antibody-glucosamine-6-phosphate-n-acetyltransferase-bha17135253","provider":"BioHippo","version":"1.0","type":"link"}