{"product_id":"got1-antibody-pair-bha10575544","title":"GOT1 Antibody Pair","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a matched capture and detection antibody pair designed for sandwich ELISA quantification of Aspartate aminotransferase, cytoplasmic in Human,Bovine,Horse. The pair consists of a capture antibody (unconjugated) and a detection antibody (biotin-conjugated), both raised in Capture: Rabbit | Detection: Rabbit, enabling a complete sandwich format without the need for additional antibody sourcing.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Capture: Polyclonal | Detection: Polyclonal — each component is independently validated for capture and detection roles in the sandwich format.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugation format:\u003c\/strong\u003e Capture: Unconjugated | Detection: Biotin conjugated — the detection antibody carries the biotin label for streptavidin-amplified signal generation; the capture antibody is unconjugated for plate coating.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype:\u003c\/strong\u003e Capture: IgG | Detection: IgG — matching isotype across capture and detection components reduces non-specific background from secondary reagents.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplication notes:\u003c\/strong\u003e We recommend using the capture antibody at a concentration of 0.1ug\/ml and the detection antibody at a concentration of 0.1ug\/ml.Optimal dilutions should be determined experimentally by the researcher.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAspartate aminotransferase, cytoplasmic\u003c\/strong\u003e (also referred to as Glutamate oxaloacetate transaminase 1,Transaminase,GOT1) is a protein target of interest in Human,Bovine,Horse systems. Researchers studying Aspartate aminotransferase, cytoplasmic typically rely on quantitative sandwich ELISA to measure protein levels in serum, plasma, cell culture supernatants, or tissue homogenates. Consult UniProt, NCBI Gene, and primary literature for current annotation of Aspartate aminotransferase, cytoplasmic expression, function, and disease relevance.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSandwich ELISA quantification:\u003c\/strong\u003e The matched pair is optimized for standard sandwich ELISA formats. The capture antibody is coated to the plate surface; after sample incubation, the biotinylated detection antibody is added, followed by streptavidin-HRP and colorimetric substrate for quantification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample types:\u003c\/strong\u003e Typically validated for serum, plasma, and cell culture supernatants. Confirm sample type compatibility with the product datasheet before use in other matrices.\u003c\/li\u003e\n\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental design\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody concentrations:\u003c\/strong\u003e We recommend using the capture antibody at a concentration of 0.1ug\/ml and the detection antibody at a concentration of 0.1ug\/ml.Optimal dilutions should be determined experimentally by the researcher.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStandard curve:\u003c\/strong\u003e Use recombinant Aspartate aminotransferase, cytoplasmic protein as a standard for absolute quantification; confirm standard purity and activity before use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Species reactivity is reported for Human,Bovine,Horse. Use in other species matrices requires empirical confirmation of antibody–antigen binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStreptavidin-HRP:\u003c\/strong\u003e Select a high-quality streptavidin-HRP conjugate and optimize its concentration to minimize background while maintaining signal sensitivity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003c!-- Sources:\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHONOLOGY LLC","offers":[{"title":"1 Pair (5*96 well plates)","offer_id":53247431442797,"sku":"CSB-EAP009679","price":1000.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/got1-antibody-pair-bha10575544","provider":"BioHippo","version":"1.0","type":"link"}