{"product_id":"gpc2-antibody-glypican-2-bha17135298","title":"GPC2 Antibody \/ Glypican 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGPC2 Antibody \/ Glypican 2 is a anti-GPC2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GPC2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe GPC2 antibody targets Glypican-2, a cell surface heparan sulfate proteoglycan encoded by the GPC2 gene. Glypican-2 is a member of the glypican family that anchors to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage. It modulates signaling pathways such as Wnt, Hedgehog, and fibroblast growth factor (FGF), thereby influencing cell proliferation, differentiation, and neural development. The GPC2 antibody provides researchers with a high-specificity reagent to study membrane-associated signaling regulation and neurodevelopmental processes.\u003cbr\u003e\u003cbr\u003eGlypican-2 is predominantly expressed in the developing nervous system, where it regulates neurite outgrowth and synaptic organization. By binding heparan sulfate chains to morphogens and growth factors, GPC2 fine-tunes signal transduction gradients essential for brain patterning. The GPC2 antibody supports detection of this proteoglycan in neuronal cultures and brain tissue, allowing exploration of its role in axonal guidance and synaptic plasticity. GPC2 also interacts with cell adhesion molecules to influence neuronal connectivity during development.\u003cbr\u003e\u003cbr\u003eRecent research has identified Glypican-2 as a cell surface oncoprotein in pediatric neuroblastoma. Overexpression of GPC2 enhances cell proliferation and tumor growth through sustained activation of oncogenic signaling pathways. The GPC2 antibody supports investigation of this function, enabling measurement of expression levels in tumor samples and evaluation of therapeutic targeting potential. Antibody-drug conjugates and immunotherapies directed against GPC2 are currently under study for selective tumor eradication.\u003cbr\u003e\u003cbr\u003eBeyond neuroblastoma, Glypican-2 contributes to neuronal regeneration and neural stem cell regulation. Its heparan sulfate chains bind multiple signaling ligands, positioning GPC2 as a key modulator of microenvironmental signaling. The GPC2 antibody helps map these ligand�receptor interactions and track expression changes during neuronal differentiation. Reduced expression of GPC2 has been linked to impaired synaptic signaling, suggesting its importance in maintaining neural circuit stability.\u003cbr\u003e\u003cbr\u003eThe GPC2 antibody is validated for western blotting, flow cytometry, and immunofluorescence, showing clear cell surface localization consistent with GPI-anchored proteins.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GPC2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GPC2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GPC2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GPC2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GPC2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287513453,"sku":"FY12395","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_888a53ef-12aa-43a1-a54d-2761cc2961aa.jpg?v=1782237014","url":"https:\/\/www.ebiohippo.com\/products\/gpc2-antibody-glypican-2-bha17135298","provider":"BioHippo","version":"1.0","type":"link"}