{"product_id":"gpd1-antibody-glycerol-3-phosphate-dehydrogenase-1-bha17135551","title":"GPD1 Antibody \/ Glycerol-3-phosphate dehydrogenase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGPD1 Antibody \/ Glycerol-3-phosphate dehydrogenase 1 is a anti-GPD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GPD1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGPD1 antibody detects Glycerol-3-phosphate dehydrogenase 1, a cytosolic enzyme that catalyzes the reversible conversion of dihydroxyacetone phosphate to glycerol-3-phosphate, linking carbohydrate metabolism with lipid biosynthesis and energy regulation. GPD1 plays a crucial role in maintaining redox balance and metabolic flexibility. The GPD1 antibody is widely used in metabolic, biochemical, and endocrinological research to study lipid metabolism, redox cycling, and adipocyte differentiation.\u003cbr\u003e\u003cbr\u003eGPD1 is encoded by the GPD1 gene located on human chromosome 12q13.12. The protein is approximately 349 amino acids long and localizes primarily in the cytoplasm. GPD1 functions in concert with its mitochondrial counterpart, GPD2, as part of the glycerophosphate shuttle that transfers reducing equivalents from NADH in the cytosol to the mitochondria for oxidative phosphorylation.\u003cbr\u003e\u003cbr\u003eThe GPD1 antibody detects a 37 kilodalton protein by western blot and shows cytosolic localization under immunofluorescence microscopy. GPD1 activity supports triglyceride synthesis in adipocytes and provides glycerol-3-phosphate as a substrate for lipid esterification. In muscle and liver, GPD1 contributes to energy metabolism by linking glycolysis to oxidative phosphorylation. Its regulation is sensitive to hormonal cues, including insulin and glucagon, and is modulated by redox state and nutrient availability.\u003cbr\u003e\u003cbr\u003eDeficiency of GPD1 results in transient hypertriglyceridemia and hepatic steatosis due to impaired lipid processing. Genetic mutations have been associated with congenital lipodystrophy and metabolic syndrome. Increased GPD1 expression in adipose tissue correlates with obesity and insulin resistance, highlighting its importance in metabolic homeostasis.\u003cbr\u003e\u003cbr\u003eBeyond energy metabolism, GPD1 influences cell signaling through regulation of NAD+\/NADH balance and oxidative stress. It plays roles in thermogenesis, adipocyte differentiation, and lipid droplet biogenesis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GPD1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GPD1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GPD1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GPD1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GPD1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047295869293,"sku":"FY12648","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_662f0a6d-0b79-4ba0-b483-7bea9d5cf23d.jpg?v=1782237030","url":"https:\/\/www.ebiohippo.com\/products\/gpd1-antibody-glycerol-3-phosphate-dehydrogenase-1-bha17135551","provider":"BioHippo","version":"1.0","type":"link"}