{"product_id":"gprc5a-antibody-g-protein-coupled-receptor-family-c-group-5-member-a-bha17135878","title":"GPRC5A Antibody \/ G-protein coupled receptor family C group 5 member A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGPRC5A Antibody \/ G-protein coupled receptor family C group 5 member A is a anti-GPRC5A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GPRC5A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGPRC5A antibody detects G-protein coupled receptor family C group 5 member A, a retinoic acid-inducible orphan receptor involved in epithelial differentiation, inflammation, and tumor suppression. The UniProt recommended name is G-protein coupled receptor family C group 5 member A (GPRC5A), also known as retinoic acid-induced protein 3 (RAI3). This membrane receptor belongs to the class C GPCR family but lacks a known endogenous ligand, functioning primarily as a signaling modulator in epithelial cells.\u003cbr\u003e\u003cbr\u003eFunctionally, GPRC5A antibody identifies a 358-amino-acid transmembrane receptor localized to the plasma membrane and endosomal compartments. GPRC5A expression is strongly induced by retinoic acid and contributes to maintaining epithelial integrity and differentiation. It modulates multiple signaling pathways including EGFR, STAT3, and NF-kappaB, thereby influencing cell growth, polarity, and immune responses. Through its regulatory functions, GPRC5A acts as a context-dependent tumor suppressor in lung, pancreatic, and colorectal tissues.\u003cbr\u003e\u003cbr\u003eThe GPRC5A gene is located on chromosome 12p13.1 and is highly expressed in lung epithelium, gastrointestinal tract, and pancreas. In normal tissues, GPRC5A helps maintain epithelial homeostasis by dampening inflammatory and growth-promoting signals. Loss of GPRC5A expression has been implicated in lung adenocarcinoma and other epithelial cancers, where its absence leads to hyperactivation of EGFR and STAT3 pathways. Conversely, aberrant overexpression in certain cancers may promote proliferation and resistance to apoptosis.\u003cbr\u003e\u003cbr\u003eIn cell signaling, GPRC5A interacts with adaptor proteins to modulate receptor internalization and trafficking. It influences the balance between pro-inflammatory and anti-inflammatory signaling, acting as a mediator of cytokine responses in epithelial cells. GPRC5A also participates in differentiation processes during lung development and regeneration following injury, making it a valuable marker for epithelial cell identity and repair mechanisms.\u003cbr\u003e\u003cbr\u003eGPRC5A antibody is used extensively in cancer biology, signal transduction, and epithelial cell research. It is applicable for immunoblotting, immunohistochemistry, and flow cytometry to evaluate receptor expression patterns and localization. In oncology, GPRC5A serves as a biomarker for tumor progression and epithelial transformation. Its detection aids in understanding the cross-talk between retinoic acid signaling and oncogenic pathways.\u003cbr\u003e\u003cbr\u003eStructurally, GPRC5A possesses seven transmembrane helices typical of GPCRs, with an extracellular N-terminus and intracellular C-terminal domain involved in signaling interactions. It lacks a known G-protein coupling profile, suggesting atypical regulatory mechanisms through scaffolding proteins.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GPRC5A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GPRC5A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GPRC5A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GPRC5A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GPRC5A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310254445,"sku":"FY12976","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_60ed5c0c-d382-4da1-89cf-3fd0315e6aa7.jpg?v=1782237074","url":"https:\/\/www.ebiohippo.com\/products\/gprc5a-antibody-g-protein-coupled-receptor-family-c-group-5-member-a-bha17135878","provider":"BioHippo","version":"1.0","type":"link"}